大豆异黄酮对阿特拉津损伤SH-SY5Y细胞的保护作用及其机制

doi:10.3969/j.issn.1004-616x.2018.01.005

癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (1): 25-30.doi: 10.3969/j.issn.1004-616x.2018.01.005

大豆异黄酮对阿特拉津损伤SH-SY5Y细胞的保护作用及其机制

李鹏, 马焜, 吴浩宇, 吴艳萍, 李百祥

哈尔滨医科大学公共卫生学院卫生毒理学教研室, 黑龙江哈尔滨 150081

收稿日期:2017-07-10 修回日期:2018-01-14 出版日期:2018-01-30 发布日期:2018-01-30
通讯作者: 李百祥,E-mail:Libaix@ems.hrbmu.edu.cn E-mail:Libaix@ems.hrbmu.edu.cn
作者简介:李鹏:E-mail:1183463484@qq.com。
基金资助:
国家自然科学基金（81273109）

Mechanism of isoflavones protection of SH-SY5Y cells from atrazine

LI Peng, MA Kun, WU Haoyu, WU Yanping, LI Baixiang

Department of Toxicology, College of Public Health, Harbin Medical University, Harbin 150081, Heilongjiang, China

Received:2017-07-10 Revised:2018-01-14 Online:2018-01-30 Published:2018-01-30

摘要/Abstract

摘要： 目的：比较大豆异黄酮、槲皮素、茶多酚、姜黄素和白藜芦醇5种植物化合物对阿特拉津（ATR）损伤神经元的保护作用及分子机制。方法：体外培养SH-SY5Y细胞，分别加入浓度为5、10、20、50、100 μmol/L的5种植物化合物，24 h后，加入250 μmol/L ATR，继续培养24 h后，CCK-8法检测细胞活力，Annexin V-FTTC/PI双染检测细胞凋亡情况，DCFH-DA探针检测活性氧（ROS）水平，Western blot检测酪氨酸氢化酶（TH）蛋白表达量，并用SPSSS 20.0进行统计学分析。结果：5种植物化合物在低剂量范围内抗ATR损伤效果显著，剂量分别为大豆异黄酮5 μmol/L、白藜芦醇5 μmol/L、槲皮素50 μmol/L、姜黄素5 μmol/L、茶多酚20 μmol/L，与其他4种植物化合物相比，大豆异黄酮抗ATR损伤SH-SY5Y细胞效果显著，尤其在5 μmol/L，可显著降低ROS水平，增加TH蛋白的表达（P < 0.05）。结论：大豆异黄酮可能通过降低SH-SY5Y细胞内ROS水平，增加TH蛋白的表达，增加细胞活力，抑制细胞凋亡。

关键词: 大豆异黄酮, 阿特拉津, 细胞凋亡, 氧自由基, 酪氨酸氢化酶

Abstract: OBJECTIVE: We tested the ability of five candidate compounds-isoflavones,resveratrol,quercetin,curcumin,and green tea polyphenols-to protect SH-SY5Y cells from atrazine (ATR) via in vitro experiments. METHODS: The cells were incubated for 24 h in the presence of various doses (5,10,20,50,and 100 μmol/L) of isoflavones,resveratrol,quercetin,curcumin,or green tea polyphenols. Cells were then cultured for 24 h with 250 μmol/L ATR. At the indicated time after treatment,the CCK-8 method was used to test for cell viability. Apoptosis was detected by using Annexin V-FITC and PI double dye,oxygen radical level was detected by using DCFH-DA method,and the protein expression of tyrosine hydroxylase (TH) by using Western blot. RESULTS: Five plant compounds showed significant anti-ATR effect at low doses,isoflavones 5 μmol/L,resveratrol 5 μmol/L,quercetin 50 μmol/L,curcumin 5 μmol/L and green tea polyphenols 20 μmol/L. Compared with other four plant compounds,the effect of isoflavones anti-ATR injury on SH-SY5Y was significant,especially in 5 μmol/L (P < 0.05). And isoflavones (5 μmol/L) could significantly reduce the level of ROS and increase the expression of TH (P < 0.05). CONCLUSION: Isoflavones could protect SH-SY5Y cells from ATR by reducing intracellular ROS levels,increasing the expression of TH and cell viability to inhibit cell apoptosis.

Key words: isoflavones, atrazine, cell apoptosis, oxygen radical, tyrosine hydroxylase

中图分类号:

R965.1

李鹏, 马焜, 吴浩宇, 吴艳萍, 李百祥. 大豆异黄酮对阿特拉津损伤SH-SY5Y细胞的保护作用及其机制[J]. 癌变·畸变·突变, 2018, 30(1): 25-30.

LI Peng, MA Kun, WU Haoyu, WU Yanping, LI Baixiang. Mechanism of isoflavones protection of SH-SY5Y cells from atrazine[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(1): 25-30.

参考文献

[1] JABLONOWSKI N D,SCHÄFFER A,BURAUEL P. Still present after all these years:persistence plus potential toxicity raise questions about the use of atrazine[J]. Environ Sci Pollut Res,2011,18(2):328-331.
[2] FORADORI C D,ZIMMERMAN A D,HINDS L R,et al. Atrazine inhibits pulsatile gonadotropin-releasing hormone (GnRH) release without altering GnRH messenger RNA or protein levels in the female rat[J]. Biol Reprod,2013,88(1).
[3] RAYNER J L,FENTON S E. Atrazine:an environmental endocrine disruptor that alters mammary gland development and tumor susceptibility[M]//Environment and Breast Cancer. New York:Springer,2011:167-183.
[4] WANG H,MU S,ZHANG F,et al. Effects of atrazine on the development of neural system of zebrafish,danio rerio[J]. BioMed Res Int,2015,1-10.
[5] POGRMIC-MAJKIC K,FA S,SAMARDZIJIA D,et al. Atrazine activates multiple signaling pathways enhancing the rapid hCG-induced androgenesis in rat Leydig cells[J]. Toxicol,2016,368:37-45.
[6] RODRIGUEZ V M,LIMON-PACHECO J H,MENDOZA-TREJO M S,et al. Repeated exposure to the herbicide atrazine alters locomotor activity and the nigrostriata dopaminergic system of the albino rat[J]. Neurotoxicology,2013,34:82-94.
[7] BARDULLAS U,GIORDANO M,RODRIGUEZ V M. Atrazine is primarily responsible for the toxicity of long-term exposure to a combination of atrazine and inorganic arsenic in the nigrostriatal system of the albino rat[J]. Neurotoxicol Teratol,2013,40:59-66.
[8] MA K,WU H Y,ZHANG B,et al. Neurotoxicity effects of atrazine-induced SH-SY5Y human dopaminergic neuroblastoma cells via microglial activation[J]. Mol Biosyst,2015,11(11):2915-2924.
[9] TSU-KUNG L,SHANG-DER C,YAO-CHUNG C,et al. Resveratrol partially prevents rotenone-induced neurotoxicity in dopaminergic SH-SY5Y cells through induction of heme oxygenase-1 dependent autophagy[J]. Mol Sci,2014,15:1625-1646.
[10] ASTRID G Z,ROSS S,ZHIXIN W,et al. Soy isoflavone glycitein protacts against beta amyloid-induced toxixity and oxidative stress in transgenic Caenorhabditis elegans[J]. BMC Neurosci,2005,6:54.
[11] NAMIKO S,MIKI H,KO F. Protective effects of quercetin against hydrogen peroxide-induced apoptosis in human neuronal SH-SY5Y cells[J]. Neurosci Lett,2011,504:223-227.
[12] FSISAL T,ANEES R,SIRAJ P,et al. ROS-dependent prostate apoptosis response-4(Par-4) up-regulation and ceramide generation are the prime signaling events associates with curcumin-induced autophagic cell death in human malignant glioma[J]. FEBS Open Biol,2014,4:763-776.
[13] SHUHONG G,ERWAN B,BAOLU Z. Protective effect of green tea polyphenols on the SH-SY5Y cells against 6-OHDA induced apoptosis through ROS-NO pathway[J]. Free Radic Biol & Med,2005,39:682-695.
[14] SONG X Y,LI J N,WU Y P,et al. Atrazine causes autophagy-and apoptosis-related neurodegenerative effects in dopaminergic neurons in the rat nigrostriatal dopaminergic system[J]. Int J Mol Sci,2015,16(6):13490-13506.
[15] SONG Y,JIA Z C,CHEN J Y,et al. Toxic effects of atrazine on reproductive system of male rats[J]. Biomed Environ Sci,2014,27(4):281-288.
[16] 何茜,李俨书,李百祥. 阿特拉津对SD大鼠TH和NURR1基因表达的影响[J]. 癌变·畸变·突变,2016,28(1):23-26.
[17] COBAN A,FILIPOV N M. Dopaminergic toxicity associated with oral exposure to the herbicide atrazine in juvenile male C57BL/6 mice[J]. J Neurochem,2007,100(5):1177-1187.
[18] 刘剑,赵菁,郑晶莹,等. 除草剂阿特拉津体内生物学毒性的研究进展[J]. 吉林大学学报:医学版,2012,38(6):1236-1240.
[19] LIU H,LO C R,CZAJA M J. NF-κB inhibition sensitizes hepatocytes to TNF-induced apoptosis through a sustained activation of JNK and c-Jun[J]. Hepatology,2002,35(4):772-778.
[20] RACHEK L I,YUZEFOVYCH L V,LEDOUX S P,et al. Troglitazone,but not rosiglitazone,damages mitochondrial DNA and induces mitochondrial dysfunction and cell death in human hepatocytes[J]. Toxicol Appl Pharmacol,2009,240(3):348-354.
[21] SKULACHEV V P. Bioenergetic aspects of apoptosis,necrosis and mitoptosis[J]. Apoptosis,2006,11(4):473-485.
[22] 马琨,吴昊宇,李鹏,等. 阿特拉津对青春期大鼠黑质多巴胺能神经元的损伤作用[J]. 癌变·畸变·突变,2017,29(4):251-155.

大豆异黄酮对阿特拉津损伤SH-SY5Y细胞的保护作用及其机制

Mechanism of isoflavones protection of SH-SY5Y cells from atrazine

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李柏茹, 秦双建, 关岚, 李闰冰, 蔡颖, 蒲桔宁, 曾明, 肖芳, 徐新云. PM_2.5对HK-2细胞氧化损伤和凋亡的影响[J]. 癌变·畸变·突变, 2021, 33(5): 360-364.
[2]	王熙凯, 孟庆贺, 高燕鲁. miR-223-3p通过调控ECT2诱导非小细胞肺癌细胞凋亡的实验研究[J]. 癌变·畸变·突变, 2021, 33(3): 187-192.
[3]	马顺鹏, 王全凯, 王苗, 宋佳阳, 乌瀚宝栎尔, 谢广云, 许建宁. 甲基丙烯酸环氧丙酯诱导16HBE细胞恶性转化过程中细胞凋亡相关lncRNA的筛选及功能研究[J]. 癌变·畸变·突变, 2021, 33(1): 1-5,31.
[4]	黄立利, 张梦云, 刘科亮, 庞定国, 刘丽达, 徐培渝. 2,4-二氯苯氧乙酸对幼龄SD大鼠肝毒性的研究[J]. 癌变·畸变·突变, 2021, 33(1): 22-27.
[5]	杨兴肖, 张雪原, 邹乃祎, 单保恩, 马鸣, 祝淑钗. HMGB1 siRNA干扰对食管鳞癌细胞放射敏感性的影响[J]. 癌变·畸变·突变, 2020, 32(4): 269-274.
[6]	华辰凤, 赵俊伟, 尚平平, 樊美娟, 谢复炜, 李翔. 不同焦油释放量卷烟全烟气气-液界面暴露致细胞毒性、炎症因子释放和细胞凋亡的研究[J]. 癌变·畸变·突变, 2020, 32(3): 182-186.
[7]	王呈谕, 刘晓璇, 李轶群, 李登科, 孙震晓. 何首乌、虎杖、大黄水提物中游离蒽醌含量的测定及对人正常肝细胞的毒性作用[J]. 癌变·畸变·突变, 2020, 32(3): 215-220.
[8]	曹宇, 张乔晔, 花蕊, 马晓玲, 汪旭, 倪娟. 白藜芦醇对人结肠细胞NCM460和SW620增殖及基因组不稳定性的影响[J]. 癌变·畸变·突变, 2019, 31(4): 283-288.
[9]	张石蕾, 由淑萍, 马晓婷, 马龙, 赵军, 刘涛. 肉苁蓉苯乙醇总苷脂质体对大鼠肝星状细胞增殖、凋亡及细胞周期的影响[J]. 癌变·畸变·突变, 2019, 31(4): 289-294.
[10]	王子依, 张露露, 孙震晓, 刘长振. 氟达拉滨与TRAIL联用诱导肺癌细胞凋亡及其作用机制[J]. 癌变·畸变·突变, 2019, 31(3): 203-207.
[11]	陈廷玉, 王东伟, 张金波, 王伟, 王景涛, 盛颜良, 卢春凤. 异烟肼通过ROS/Caspase-3信号通路诱导L-02细胞凋亡及槲皮素的保护作用[J]. 癌变·畸变·突变, 2019, 31(1): 53-57,68.
[12]	刘梦琪, 张文文, 陈晓伟, 沈孝兵. PD98059抑制MAPK/ERK信号通路对胃癌细胞生物学功能的影响[J]. 癌变·畸变·突变, 2019, 31(1): 15-21.
[13]	孙凤霞, 孟醒, 孙蓉丽, 张娟, 尹立红, 浦跃朴. HIF-1α高表达对苯代谢物诱导K562细胞毒性的影响[J]. 癌变·畸变·突变, 2018, 30(1): 47-51,57.
[14]	高晓洁, 孔璐, 薛玉英. 纳米镍对大鼠睾丸生精-支持共培养细胞的凋亡作用及机制研究[J]. 癌变·畸变·突变, 2018, 30(1): 8-13,19.
[15]	马焜, 吴昊宇, 李鹏, 王丽, 李百祥. 阿特拉津对青春期大鼠黑质区多巴胺能神经元的损伤作用[J]. 癌变·畸变·突变, 2017, 29(4): 251-255.