Carcinogenesis, Teratogenesis & Mutagenesis ›› 2018, Vol. 30 ›› Issue (4): 270-274.doi: 10.3969/j.issn.1004-616x.2018.04.005

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Reactive oxygen species scavenging on vitamin E succinate-induced endoplasmic reticulum stress response in SGC-7901 cells

HUANG Xiaoli1, WU Kun2, ZHAO Shasha1   

  1. 1. Department of Nutrition, Qilu Hospital of Shandong University, Jinan 250012, Shandong;
    2. Department of Nutrition and Food Hygiene, Harbin Medical University, Harbin 150081, Heilongjiang, China
  • Received:2017-11-08 Revised:2017-12-26 Online:2018-07-30 Published:2018-07-30

Abstract: OBJECTIVE: To investigate effects of reactive oxygen species (ROS) scavenging on vitamin E succinate(VES)-induced endoplasmic reticulum stress response (ERS) in human gastric cancer SGC-7901 cells. MATERIALS: SGC-7901 human gastric cancer cells were treated with 1,5,10,20 mmol/L N-acetyl-L-cysteine (NAC) for 2 h and then with 20 μg/mL VES for an additional 12 h;ROS production was measured using confocal microscopy. In addition,cells were pretreated with 20 mmol/L NAC for 2 h and then with 20 μg/mL VES for an additional 12 h;there after,ROS production was measured using flow cytometry. In addition,cells were pretreated with 20 mmol/L NAC for 2 h and subsequently treated with 20 μg/mL VES for 15 h and 24 h,CHOP and GRP78 were determined by RT-PCR and Western blot. RESULTS: Pretreatment with NAC drastically decreased VES-induced ROS generation in SGC-7901 cells (P < 0.05). NAC significantly inhibited induction of CHOP and GRP78 mRNA and protein expression in VES-treated SGC-7901 cells (P < 0.05). CONCLUSION: ER stress response is an event downstream of the oxidative stress induced by VES in SGC-7901 cells.

Key words: reactive oxygen species, vitamin E succinate, endoplasmic reticulum stress, gastric cancer cells

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