SNP芯片联合质谱筛检胃癌相关基因3'UTR区SNPs的病例-对照研究

doi:10.3969/j.issn.1004-616x.2018.05.006

Abstract

Abstract: OBJECTIVE:To explore the new miRNA target gene 3'untranslated region (3'UTR) single nucleotide polymorphism (SNPs) among digestive tumor associated genes, and their association with gastric carcinogenesis. METHODS:The 1:1 matched case-control study was conducted. SNP microarray was used to detect the SNP loci of 3'UTR region of digestive tract tumor-associated genes in 96 patients with gastric adenocarcinoma and 96 healthy controls in the high incidence area of gastric cancer in Xianyou County,Fujian Province. Time-of-flight mass spectrometry (TFMS) was used to detect the SNP loci in 622 patients with gastric cancer and 622 healthy individuals. RESULTS:SNP chip and bioinformatics analyses selected EGFR rs884225,EGFR rs10277413,FAS rs1468063,MSH2 rs17502941 and MSH2 rs11125144 as candidate genes. The SNP genotyping of 622 candidate genes in the control group showed no correlation between the above 5 loci and susceptibility to gastric cancer. Further stratification analyses showed that the genotype (CT+TT) with T allele in EGFR rs884225 was associated with cardiac cancer (OR=0.67,95% CI:0.46-0.99), while other loci were not associated with the risk of cardiac cancer and non-cardiac cancer (P > 0. 05). CONCLUSION:The rs884225 polymorphism of EGFR gene 3'untranslated region is associated with the occurrence and development of gastric cancer in Xianyou County,a high incidence area of gastric cancer in Fujian Province. T allele may be a genetic protective factor for gastric cancer.

Key words: 3'UTR, target genes, single nucleotide polymorphism, gastric cancer

CLC Number:

R735.2

HAN Renjie, WU Chuancheng, LIU Baoying, GUO Saixiong, CHEN Yu. A case-control study of SNPs in the 3'UTR region of digestive tumor associated genes-detected by SNP chip combined with time-of-flight mass spectrometry[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(5): 359-364.

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A case-control study of SNPs in the 3'UTR region of digestive tumor associated genes-detected by SNP chip combined with time-of-flight mass spectrometry

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