SF1a-PRLR和ΔS2 SF1a-PRLR对乳腺癌MCF-7细胞增殖和microRNA表达的影响

doi:10.3969/j.issn.1004-616x.2020.03.009

Abstract

Abstract: OBJECTIVE: To investigate the effects of SF1a-PRLR and its variant ΔS2 SF1a-PRLR over-expression on proliferation and microRNA expression in human breast cancer cells (MCF-7). METHODS: SF1a-PRLR and ΔS2 SF1a-PRLR cDNAs were recombined separately into lentivirus by using homologous recombination strategy,and the viruses carrying different genes (including SF1a-PRLR,or ΔS2 SF1a-PRLR cDNA) or not carrying any target gene (control) were transfected into MCF-7 cells,respectively. The stably transfected cells including MCF7-con (control group),MCF7-SF1a-PRLR (SF1a),and MCF7-ΔS2 SF1a-PRLR (ΔS2 SF1a) were obtained through multiple screening with puromycin. Total RNAs were extracted using Trizol reagent,and high-throughput sequencing of small RNAs were performed. In addition, separate sets of the cells were tested using the MTS cell proliferation assay. RESULTS: Cell proliferation assay results show that at 48 h,the D(492) values of the control,SF1a and ΔS2 SF1a groups were 1.85±0.29,2.24±0.26 and 2.57±0.23,with significant differences among them (all P < 0.05). Furthermore, sequencing data show that there were significant differences on microRNA expression among the groups. SF1a induced 176 microRNAs expression changes(32 were up-regulated and 144 were down-regulated) and ΔS2 SF1a induced 206 microRNAs expression changes (52 were up-regulated and 154 were down-regulated). For comparison,4 microRNAs (miR-4454,miR-215-5p,miR-622 and miR-797) were up-regulated and 1 (miR-210-5p) was down-regulated from ΔS2 SF1a-PRLR over-expression. The relative expressions of miR-210-5p in the control,SF1a and ΔS2 SF1a groups were 66.18、31.67 and 13.07,with significant differences among the three groups (all P < 0.05). CONCLUSION: Both SF1a-PRLR and ΔS2 SF1a-PRLR over-expression promoted proliferation of breast cancer cells (MCF-7),and significantly affected the expression of microRNAs in a similar way for most of the detected genes. Several microRNAs such as miR-4454,etc. are affected in a different mode by SF1a PRLR and ΔS2 SF1a-PRLR.

Key words: breast cancer, SF1a-PRLR, ΔS2 SF1a-PRLR, microRNA, differential expression

CLC Number:

ZHANG Huijuan, HUANG Dayuan, ZHANG Jie, TANG Haiou, XIE Anxin, TAN Dunyong. Effects of SF1a-PRLR and ΔS2 SF1a-PRLR on proliferation and microRNA expressionin MCF-7 breast cancer cell[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 203-208.

References

[1] 谭敦勇, 彭湘萍. 催乳素受体研究[J]. 生理科学进展, 2012, 43(1):17-23.
[2] TAN D Y, HUANG K T, UEDA E, et al. S2 deletion variants of human PRL receptors demonstrate that extracellular domain conformation can alter conformation of the intracellular signaling domain[J]. Biochemistry, 2008, 47(1):479-489.
[3] SAKAMOTO N, NAITO Y, OUE N, et al. microRNA-148a is downregulated in gastric cancer, targets MMP7, and indicates tumor invasiveness and poor prognosis[J]. Cancer Sci, 2014, 105(2):236-243.
[4] KONG X X, LV Y R, SHAO L P, et al. HBx-induced MiR-1269b in NF-κB dependent manner upregulates cell Division cycle 40 homolog (CDC40) to promote proliferation and migration in hepatoma cells[J]. J Transl Med, 2016, 14(1):189.
[5] WANG L Y, LI B, JIANG H H, et al. Inhibition effect of miR-577 on hepatocellular carcinoma cell growth via targeting β-catenin[J]. Asian Pac J Trop Med, 2015, 8(11):923-929.
[6] WANG X Y, REN Y L, YANG X Y, et al. MiR-190a inhibits epithelial-mesenchymal transition of hepatoma cells via targeting the long non-coding RNA treRNA[J]. FEBS Lett, 2015, 589(24 Pt B):4079-4087.
[7] ZHAO Y, LU G F, KE X Q, et al. MiR-488 Acts as a tumor suppressor gene in gastric cancer[J]. Tumour Biol, 2016, 37(7):8691-8698.
[8] 苏雪婷. miR-138、miR-30家族新靶标的确证及生物学功能研究[D]. 北京:中国人民解放军军事医学科学院, 2013.
[9] CHAI J, WANG S, HAN D L, et al. microRNA-455 inhibits proliferation and invasion of colorectal cancer by targeting RAF proto-oncogene serine/threonine-protein kinase[J]. Tumour Biol, 2015, 36(2):1313-1321.
[10] 王娅南, 魏亚宁, 徐芳, 等. miR-551b在人胃癌组织中的表达及对胃癌细胞凋亡的影响[J]. 华中科技大学学报:医学版, 2017, 46(4):386-391.
[11] 李勇传, 石永杰, 嘉红云, 等. MiR-105抑制肝癌细胞增殖能力的实验研究[J]. 海南医学, 2017, 28(7):1038-1040.
[12] LIANG J J, DEJULIUS K L, JARRAR A, et al. Potential tumor suppressor miR-1247 is down-regulated in CpG island methylator phenotype colon cancers[J]. J Am Coll Surg, 2012, 215(3):S11.
[13] 钱建升, 窦建卫, 李宇. miR-338对人肝癌细胞增殖及迁移的影响[J]. 现代肿瘤医学, 2016, 24(19):3001-3004.
[14] 杨琦, 吴彩云, 王成, 等. miR-651和miR-708作为肾细胞癌潜在的分子标志物通过调控MTMR3、RAP1B和MTF1参与肾细胞癌的发生发展[C]//中华医学会检验医学分会. 中华医学会第八次全国中青年检验医学学术会议报告集. 济南, 2014.
[15] 黄光成. 乳腺癌中P53的表达及其与miR-449a的调控机制研究初探[D]. 南充:川北医学院, 2017.
[16] DONG D, GONG Y B, ZHANG D B, et al. MiR-874 suppresses the proliferation and metastasis of osteosarcoma by targeting E2F3[J]. Tumour Biol, 2016, 37(5):6447-6455.
[17] ZHAO J, NIE Y Q, WANG H, et al. MiR-181a suppresses autophagy and sensitizes gastric cancer cells to cisplatin[J]. Gene, 2016, 576(2 Pt 2):828-833.
[18] CHEN Z F, LIU K P, LI L, et al. MiR-215 promotes cell migration and invasion of gastric cancer by targeting Retinoblastoma tumor suppressor gene 1[J]. Pathol Res Pract, 2017, 213(8):889-894.
[19] BRAUN C J, ZHANG X, SAVELYEVA I, et al. P53-Responsive micrornas 192 and 215 are capable of inducing cell cycle arrest[J]. Cancer Res, 2008, 68(24):10094-10104.
[20] SONG C S, LU P F, SHI W C, et al. MiR-622 functions as a tumor suppressor and directly targets E2F1 in human esophageal squamous cell carcinoma[J]. Biomedecine Pharmacother, 2016, 83:843-849.
[21] 成林. miR-210抑制食管Eca109细胞生长的机制研究[D]. 石家庄:河北医科大学, 2014.
[22] CHEN W Y, LIU W J, ZHAO Y P, et al. Induction, modulation and potential targets of miR-210 in pancreatic cancer cells[J]. HBPD INT, 2012, 11(3):319-324.
[23] CAMPS C, BUFFA F M, COLELLA S, et al. Hsa-miR-210 Is induced by hypoxia and is an independent prognostic factor in breast cancer[J]. Clin Cancer Res, 2008, 14(5):1340-1348.
[24] TSUCHIYA S, FUJIWARA T, SATO F, et al. microRNA-210 regulates cancer cell proliferation through targeting fibroblast growth factor receptor-like 1(FGFRL1)[J]. J Biol Chem, 2011, 286(1):420-428.
[25] GIANNAKAKIS A, SANDALTZOPOULOS R, GRESHOCK J, et al. MiR-210 links hypoxia with cell cycle regulation and is deleted in human epithelial ovarian cancer[J]. Cancer Biol Ther, 2008, 7(2):255-264.

Effects of SF1a-PRLR and ΔS2 SF1a-PRLR on proliferation and microRNA expressionin MCF-7 breast cancer cell

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