维生素E琥珀酸酯对人食管癌TE-1和Eca-109细胞增殖及凋亡的影响

doi:10.3969/j.issn.1004-616x.2015.05.006

Abstract

Abstract:

OBJECTIVE: To study the effects of vitamin E succinate(VES) on proliferation, apoptosis and expressions of associated protein of human esophageal carcinoma cell line Eca-109 and TE-1．METHODS:Eca-109 and TE-1 human esophageal carcinoma cells were cultured in vitro. Cells were treated with different concentrations of VES for 24 h. MTT was used to measure the proliferation of cells. Morphological changes of cells were examined by inverted microscopy. Apoptosis was assessed by DAPI staining. The expression of poly ADP-ribose polymerase(PARP) of two cell lines among different concentration groups was examined by Western blotting．RESULTS:The inhibitory rate increased gradually with increasing concentration of vitamin E succinate (P<0.05). The number of dead cells decreased with increasing concentration of VES. Apoptosis appeared in different degrees in cells with increasing concentration of VES. The cleavage of PARP protein in two cell lines also increased gradually. CONCLUSION:Vitamin E succinate could inhibit proliferation and induce apoptosis of Eca-109 and TE-1 cell lines．

Key words: vitamin E succinate, human esophageal carcinoma cells, apoptosis, proliferation

CLC Number:

R730.2

CHEN Haijun, LIU Yuqin, LI Yuze, WANG Xin, LI Nan, LI Dianzhong. Vitamin E succinate inhibits proliferation and induces apoptosis in human esophageal carcinoma cells TE-1 and Eca-109[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 357-360.

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Vitamin E succinate inhibits proliferation and induces apoptosis in human esophageal carcinoma cells TE-1 and Eca-109

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