miR-19a和miR-19b在骨肉瘤中的表达及其临床意义

doi:10.3969/j.issn.1004-616x.2017.01.011

Abstract

Abstract:

OBJECTIVE: To investigate the expression of miR-19a and miR-19b in osteosarcoma and adjacent non-cancerous tissues,and their correlation with clinicopathologic characteristics. METHODS: 32 cases of osteosar-coma tissues and paired adjacent non-cancerous tissues were collected. qPCR was used to detect the expressions of miR-19a and miR-19b in the two tissues. Correlation between expression of the miRNA and clinical pathologic changes was performed. RESULTS: Expression of miR-19a and miR-19b was significantly up-regulated in osteosarcoma tissues as compared with the adjacent non-cancerous tissues. Their expression nucleic acid levels was positively and significantly correlated with pathologic gradings (all P < 0.05). The expression of the miR-19a was also positive correlated with clinical stage of osteosarcoma and related to the prognosis (r=0.685,P < 0.05). Multivariate analyses suggested that miR-19a expression was an independent predictor for overall survival (P=0.037). CONCLUSION: Our study showed that miR-19a and miR-19b may play an important role in the tumorigenesis and progression of osteosarcoma. In particular,miR-19a may serve as a prognostic marker and therapeutic target for osteosacoma.

Key words: osteosarcoma, miR-19a, miR-19b, prognosis

CLC Number:

R711.74

HU Weixin, ZHAO Zhiwei. Expression of miR-19a and miR-19b and their clinical significance in osteosarcoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(1): 55-59.

References

[1] NUGENT M. microRNA and bone cancer[J]. Adv Exp Med Biol,2015,889:201-230.

[2] KAFCHINSKI L A,JONES K B. MicroRNAs in osteosar-comagenesis[J]. Adv Exp Med Biol,2014,804:119-127.

[3] ALLEN-RHOADES W,KURENBEKOVA L,SATTERFIELD L,et al. Cross-species identification of a plasma microRNA signature for detection,therapeutic monitoring,and prognosis in osteosarcoma[J]. Cancer Med,2015,4(7):977-988.

[4] ZIYAN W,SHUHUA Y,XIUFANG W,et al. MicroRNA-21 is involved in osteosarcoma cell invasion and migration[J]. Med Oncol,2011,28(4):1469-1474.

[5] TSUCHIDA A,OHNO S,WU W,et al. miR-92 is a key oncogenic component of the miR-17-92 cluster in colon cancer[J]. Cancer Sci,2011,102(12):2264-2271.

[6] MENDELL J T. miRiad roles for the miR-17-92 cluster in development and disease[J]. Cell,2008,133(2):217-222.

[7] HE L,THOMSON J M,HEMANN M T,et al. A microRNA polycistron as a potential human oncogene[J]. Nature,2005, 435(7043):828-833.

[8] WU W,TAKANASHI M,BORJIGIN N,et al. MicroRNA-18a modulates STAT3 activity through negative regulation of PIAS3 during gastric adenocarcinogenesis[J]. Br J Cancer, 2013,108(3):653-661.

[9] MATSUBARA H,TAKEUCHI T,NISHIKAWA E,et al. Apoptosis induction by antisense oligonucleotides against miR-17-5p and miR-20a in lung cancers overexpressing miR-17-92[J]. Oncogene,2007,26(41):6099-6105.

[10] MORIMURA R,KOMATSU S,ICHIKAWA D,et al. Novel diagnostic value of circulating miR-18a in plasma of patients with pancreatic cancer[J]. Br J Cancer,2011,105(11):1733-1740.

[11] HUANG G,NISHIMOTO K,YANG Y,et al. Participation of the Fas/FasL signaling pathway and the lung microenvironment in the development of osteosarcoma lung metastases[J]. Adv Exp Med Biol,2014,804:203-217.

[12] ZHU H,HAN C,LU D,et al. miR-17-92 cluster promotes cholangiocarcinoma growth:evidence for PTEN as downstream target and IL-6/Stat3 as upstream activator[J]. Am J Pathol, 2014,184(10):2828-2839.

[13] WANG F,LI T,ZHANG B,et al. MicroRNA-19a/b regulates multidrug resistance in human gastric cancer cells by targeting PTEN[J]. Biochem Biophys Res Commun,2013,434(3):688-694.

[14] XU X L,JIANG Y H,FENG J G,et al. MicroRNA-17, microRNA-18a,and microRNA-19a are prognostic indicators in esophageal squamous cell carcinoma[J]. Ann Thorac Surg, 2014,97(3):1037-1045.

[1]	PENG Youbin, LIN Hao, LI Ming, HUANG Haihua. Expression of TPX2 and its clinicalsignificance in non-muscle-invasive bladder cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2022, 34(1): 30-34.
[2]	JIAO Wenpeng, HOU Lin, CUI Meijuan, JIAO Wenjing, MA Ming, ZHANG Jinyan. Expression of miR-93-5p and miR-106b-5p in alcohol-related liver cancer and its clinical significance [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 430-434.
[3]	GAO Xiaobin, WU Xueliang, WANG Shengjie, SUN Guangyuan, WANG Wenjing, LIANG Feng, ZHAO Yifeng, LIU Zhenxian, ZHANG Yingchun. Expression of TRIM59 and its correlations with clinicopathological features in colorectal cancers [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(6): 446-450.
[4]	JIAO Wenjing, ZHANG Jinyan, MA Ming, GUO Xiujuan, FENG Junhua, SHAO Junguo, JIAO Wenpeng. Expression and clinical significance of MTDH and FoxM1 in hepatocellular carcinomas [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(5): 349-353,359.
[5]	ZHANG Shimin, ZHAO Ruijun. Expression of protein tyrosine phosphatase receptor J in breast cancer tissues and its relationship with clinical prognosis [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 276-279,285.
[6]	LIU Xiaoxia, LI Weixia, LI Zhijia, SU Zhenjun, DUAN Xiaohui, HAN Weiwen, WANG Yunxiao, HE Xiaolei, Lu Linlin, JIA Jinhai. Correlations between glutathione peroxidase in serum and clinicopathological outcomes of advanced non-small cell lung cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(4): 286-290,295.
[7]	ZHANG Yuling, WU Jundong, PANG Dongyue, CHEN Chunfa. Changes of Ki67 expression before and after neoadjuvant chemotherapy as a predictor for therapeutic response and rognosis in breast cancers [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2021, 33(3): 230-235,245.
[8]	SHI Hailin, HE Tianji, LIU Feng, CAI Menghui, GE Bo. Prognostic value of TCGA-database mutation burden in muscle-invasive bladder cancers [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 430-437,443.
[9]	DING Yawen, JIAO Wenjing, WANG Pengyu, LU Gang, MA Ming. Immunophenotype characteristics of plasma cells and clinical significance in multiple myeloma patients [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(6): 464-468,489.
[10]	SHAO Junguo, JIAO Wenjing, WANG Xuexiao, ZHANG Jinyan, MA Ming. Clinical significance of mi-429 and Bmi-1 mRNA expressions in lung carcinoma tissues [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(5): 369-373.
[11]	Lü Yanping, WU Chuancheng, YANG Shuangfeng, HE Chenzhou, HAN Renjie, YAN Wei, LIU Baoying. Relationship between differentially expressed miRNA-SNPs and prognosis of gastric cancer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(5): 380-386.
[12]	GUO Xiujuan, DU Yanyan, YAN Xi, ZHAO Xuetao, HAN Lina, WU Yipeng, WANG Xuexiao, MA Ming. Expression and clinical significance of CREB in esophageal squamous cell carcinoma tissues [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(5): 387-390,394.
[13]	YANG Li, ZHANG Mengxian, ZHANG Wei, XIONG Yingyou, CHEN Shiyong, FANG Chengxiang. Data mining to investigate expression and significance of aurora kinase A in esophageal carcinoma [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2020, 32(3): 221-228.
[14]	CHEN Xiuqiong, MENG Fanqiao, XIONG Hua, WANG Yali, ZHOU Yangmei, TANG Wenhua, ZOU Yanmei. Investigation and evaluation of gastric cancer core genes using bioinformatics [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(4): 308-314.
[15]	GAO Jie, ZHANG Xiao, WEI Chao. Involvement of differentially expressed miRNA in prognosis of pancreatic cancers [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(3): 173-179.

Expression of miR-19a and miR-19b and their clinical significance in osteosarcoma

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments