DNA糖基化酶1对幽门螺杆菌致胃上皮细胞DNA损伤的保护作用

doi:10.3969/j.issn.1004-616x.2018.02.003

Abstract

Abstract: OBJECTIVE: DNA glycosylase 1 (OGG1) is a critical factor involved in oxidative DNA damage repair,the aim of this study was to investigate the role of OGG1 in Helicobacter pylori (HP)-induced DNA damage in gastric epithelial cells (GES-1). METHODS: Cultured cells were divided into four treatment groups:control,HP infection,OGG1 siRNA,HP infection plus OGG1 siRNA. Human gastric epithelial cells (GES-1) were infected with HP at 24 h after OGG1 inhibition through the use of RNA interference. 24 h and 48 h after HP infection,cytotoxicity was evaluated based on viability using the CCK-8 assay and lactate dehydrogenase (LDH) release. DNA damage was measured using the phosphorylated H2AX (γH2AX) and the comet assays. Apoptosis was determined by TUNEL assay and western blot against poly-ADP-ribose polymerase (PARP). RESULTS: Compared to the control,no significant differences were found in cell viability,LDH release,DNA damage,γH2AX foci formation,TUNEL-positive cells and the expression levels of cleaved PARP in HP infection and OGG1 siRNA groups. However,compared to the control and the HP infection groups,HP infected cells with OGG1 deficiency showed reduction in cell viability and increased LDH release. Moreover,HP infection in OGG1 deficient cells induced DNA damage and γH2AX foci formation,elevated TUNEL-positive cells and expression of cleaved PARP. After statistical analysis,there were significant differences in the above indexes (P < 0.05 or 0.01). CONCLUSION: These findings indicate that OGG1 played a protective role in HP-induced DNA damage in gastric epithelial cells and the information may provide a new strategy for preventing HP infection-related gastric diseases.

Key words: DNA glycosylase 1, Helicobacter pylori, DNA damage, γH2AX focus, oxidative stress

CLC Number:

R730.231

WANG Yuan, DENG Xiaofei. Effect of DNA glycosylase OGG1 against Helicobacter pylori-induced DNA damage in gastric epithelial cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(2): 92-97,102.

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Effect of DNA glycosylase OGG1 against Helicobacter pylori-induced DNA damage in gastric epithelial cells

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