氯喹通过激活P38MAPK通路诱导人肝癌HepG2细胞凋亡

doi:10.3969/j.issn.1004-616x.2018.06.003

Abstract

Abstract: OBJECTIVE: To investigate effects of chloroquine on cell proliferation and apoptosis of a hepatocellular carcinoma cell line,HepG2. METHODS: Cultured HepG2 cells were treated with various concentrations of chloroquine (0,10,20 and 40 μmol/L). Cell viability was determined by the MTT assay. Morphological changes in cell nuclei was detected using fluorescence microscope at 24 h after treatment. Cell apoptosis was detected by flow cytometry. Expression levels of Caspase-3,Bcl-2,Bax,P-P53,P38MAPK and P-P38MAPK were performed using Western blot analyses. RESULTS: After treatment with 10-40 μmol/L chloroquine for 24,48 and 72 h,proliferation of HepG2 cells was significantly inhibited compared with the control group (P < 0.05). In addition,treatment with 10,20 and 40 μmol/L led to a significant increase in nuclear concentration and shrinkage. Flow cytometry analyses showed that 10,20 and 40 μmol/L treatment significantly induced apoptosis (P < 0.05). Increased in protein expression of P-P53,P-P38MAPK,cleaved Caspase-3 and Bax was detected in the 20 and 40 μmol/L groups while Caspase-3 and P38MAPK did not change. Expression of Bcl-2 was decreased after treatment with 20 and 40 μmol/L. CONCLUSION: Chloroquine inhibited HepG2 cell growth and induced apoptosis. The underlying mechanism might involve the P38MAPK signaling pathway.

Key words: chloroquine, hepatocellular carcinoma, apoptosis, P38MAPK pathway, P53

CLC Number:

R730.1

DING Wenping, LU Yuanyuan, TONG Wenxia, ZHOU Aibin, KONG Xiang, CHEN Yihong. Induction of apoptosis in HepG2 hepatocellular carcinoma cells by chloroquine via the P38MAPK pathway[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(6): 430-434.

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Induction of apoptosis in HepG2 hepatocellular carcinoma cells by chloroquine via the P38MAPK pathway

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