TGF-β1对UVB辐射诱导人角质形成细胞miRNA和环氧合酶-2表达的影响及机制

doi:10.3969/j.issn.1004-616x.2019.05.001

Abstract

Abstract: OBJECTIVE:To study the effect of TGF-β1 on expression of miRNA and the inflammatory protein,cyclooxygenase-2 (COX-2),in human keratinocytes (HaCaT) after UVB irradiation. METHODS:10 ng/μL of TGF-β1 was added to cultured HaCaT cells for 48 h and then irradiated with UVB:0,10,20,30 mJ/cm₂ UVB and different time (4,12 and 24 h) after 30 mJ/cm₂ UVB irradiation. Real-time fluorescent quantitative PCR was used to detected expression levels of miRNA-23a and let-7c 6 h after irradiation. Expression levels of COX-2 and caspase-3 were detected by Western blot at different time (0,2,4,8,12,24 and 48 h) after 30 mJ/cm₂ UVB irradiation. RESULTS:Expression of miR-23a was increased and let-7c was generally down-regulated. Expression of COX-2 was significantly increased (P < 0.05),and cleaved-caspase3 decreased gradually at 12-48 h after irradiation. CONCLUSION:The data indicate that TGF-β could regulate the expression levels of miRNA and COX-2 which have been shown to play a role in cell proliferation,infiltration,inflammation and anti-apoptosis.

Key words: TGF-β, UVB, miRNA, COX-2, inflammation

CLC Number:

R811.5

LI Yuwen, LI Chen, FENG Jiangbin, FAN Li, LIU Jianxiang, LIU Qingjie, TIAN Mei. Effect of UVB irradiation on TGF-β1,and expression of miRNA and cyclooxygenase-2 in human keratinocytes[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2019, 31(5): 341-346.

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Effect of UVB irradiation on TGF-β1,and expression of miRNA and cyclooxygenase-2 in human keratinocytes

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