稀土镧离子对大肠杆菌基因组DNA的影响

doi:10.3969/j.issn.1004-616x.2006.02.010

癌变·畸变·突变 ›› 2006, Vol. 18 ›› Issue (2): 116-118.doi: 10.3969/j.issn.1004-616x.2006.02.010

稀土镧离子对大肠杆菌基因组DNA的影响

汪承润1;陈华波1;杨　帆2;郭汉卿2;王子尧2;丁铁林2

1.淮南师范学院化学生物系,安徽　淮南 232001;2.淮南师范学院化学生物系02级生命科学专业　安徽　淮南　232001

收稿日期:2005-07-04 修回日期:2005-09-20 出版日期:2006-03-30 发布日期:2006-03-30
通讯作者: 汪承润

Effects of Lanthanide Ions in Rare Earths on Genomic DNA of E.coli

WANG Cheng-run1; CHEN Hua-bo1; Yang Fan2; GUO Han-qing2; WANG Zi-yao2; DING Tie-lin2

1.Department of Chemistry and Biology, Huainan Normal College, Huainan 232001,Anhui,China; 2. Undergraduates of Year 2002, Department of Chemistry and Biology, Huainan Normal College, Huainan 232001,Anhui,China

Received:2005-07-04 Revised:2005-09-20 Online:2006-03-30 Published:2006-03-30
Contact: WANG Cheng-run

摘要/Abstract

摘要： 背景与目的: 通过研究硝酸镧溶液对大肠杆菌基因组DNA的作用，探讨稀土镧离子对原核生物的遗传毒性效应。材料与方法: 用含硝酸镧分别为10、40、80、160、320 mg/L的LB培养基培养大肠杆菌,以不添加硝酸镧组作为对照,于培养10 h,15 h和20 h后分别采样提取总DNA,琼脂糖凝胶电泳; 并用含硝酸镧分别为50、200、400、800、1 600 mg/L的LB培养基培养大肠杆菌, 未添加硝酸镧组作为对照, 于培养30 h后分别采样提取总DNA,琼脂糖凝胶电泳。结果: 高剂量镧离子组大肠杆菌基因组DNA出现降解或交联现象，电泳带型荧光减弱，不清晰或滞留于点样孔不能泳出。结论: 镧离子对大肠杆菌基因组DNA可能具有降解或交联作用。

关键词: 稀土, 镧离子, 大肠杆菌, 基因组DNA, DNA降解作用, DNA 交联作用

Abstract: BACKGROUND ＆ AIM: To investigate genotoxic effect of lanthanide ions on prokaryote by observing the effects of lanthanide nitrate on genomic DNA of E.coli. MATERIALS AND METHODS: TreatmentⅠ: E.coli was cultured in LB medium with different concentrations of lanthanide nitrate (10,40,80,160,320 mg/L), LB medium without lanthanide nitrate was the control. Genomic DNA was extracted at 10, 15, and 20 h. Then Agarose gel electrophoresis was performed Treat mentⅡ: E.coli was cultwred in LB medium with different concentrations of lanthanide nitrate (50,200,400,800,1 600 mg/L) LB medium without lanthanide nitrate was the control. Genomic DNA was extracted at 30 h. Then Agarose gel electrophoresis was performed RESULTS: DNA degradation or cross-linkage was produced by high concentrations of lanthanide ions. CONCLUSION: Lanthanide ions affected degradation and crosslinking on genomic DNA of E.coli.

Key words: rare earths, lanthanide ions, E.coli, genomic DNA, DNA degradation, DNA crosslink

中图分类号:

Q255
Q503

汪承润, 陈华波, 杨　帆, 郭汉卿, 王子尧, 丁铁林. 稀土镧离子对大肠杆菌基因组DNA的影响[J]. 癌变·畸变·突变, 2006, 18(2): 116-118.

WANG Cheng-run, CHEN Hua-bo, Yang Fan, GUO Han-qing, WANG Zi-yao, DING Tie-lin. Effects of Lanthanide Ions in Rare Earths on Genomic DNA of E.coli[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2006, 18(2): 116-118.

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稀土镧离子对大肠杆菌基因组DNA的影响

Effects of Lanthanide Ions in Rare Earths on Genomic DNA of E.coli

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