癌变·畸变·突变 ›› 2000, Vol. 12 ›› Issue (1): 47-048.doi: 10.3969/j.issn.1004-616x.2000.01.015

• 论著 • 上一篇    下一篇

应用冰冻切片标本检测乳腺癌c - erbB- 2 基因扩增的研究

吴建中,沈宗丽,陈森清,薛开先   

  1. 江苏省肿瘤防治研究所,江苏南京　210009
  • 收稿日期:1999-07-03 修回日期:1999-09-01 出版日期:2000-01-30 发布日期:2000-01-30

STUDY ON C - ERBB - 2 GENE AMPLIFICATION IN FROZEN SECTIONS OF BREAST CANCER

WU Jian-zhong, SHEN Zong-li, CHEN Sen-qing, XUE Kai-xian   

  1. Jiangsu Institute of Cancer Research, Nanjing 　210009, China
  • Received:1999-07-03 Revised:1999-09-01 Online:2000-01-30 Published:2000-01-30

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摘要： 目的与方法:c - erbB - 2 基因的扩增与乳腺癌的治疗和预后有一定的关系。为了能够快速、简便、准确的检测乳癌中c- erbB - 2 基因的扩增,我们将靶基因与参照基因设在同一条染色体上,同时选用快速冰冻切片的剩余组织作d - PCR 实验。结果:研究结果表明,71 例乳腺癌中共有19 例c - erbB - 2 基因的扩增,扩增率为26. 7 %。与我们过去的研究相比,c - erbB -2 基因扩增检出率有所提高。结论:本文认为,引物设计合理、选材得当,能够提高c - erbB - 2 基因扩增的准确检出率。

关键词: 聚合酶链式反应, 乳腺癌, c - erbB - 2 基因

Abstract: Purpose : The amplification of HER - 2/ neu may have prognostic value in breast cancer. Methods : In this study , we chose the reference gene on the same chromosome as the target gene , and used the differential polymerase chain reaction (d - PCR) to assess the presence of c - erbB - 2 gene amplification in frozen sections of breast cancer. Results : The result s showed that there were 19 of 71 cases of breast cancer were detected amplification of c - erbB - 2 gene , the detectable rate was 26. 7 % , which was higher than our previous reports. Conclusion : the author regards that the chose of primer and the specimens cont ribute to the improvement of the detectable rate.

Key words: differential polymerase chain reaction (d - PCR), breast cancer, c - erbB - 2 gene

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