癌变·畸变·突变 ›› 2000, Vol. 12 ›› Issue (4): 202-204.doi: 10.3969/j.issn.1004-616x.2000.04.003

• 论著 • 上一篇    下一篇

HBV表面抗原DNA疫苗对小鼠脏器影响的实验研究

方玲，姚玉成 ，案宏彪 ，谢庆东 ．赵书民 ，王忠华 ，余宏宇 ，胡以平   

  1. 1广西医科大学生物学教研室．广西南宁530021；2第一军医大学细胞生物学教研室．上海200433
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2000-10-30 发布日期:2000-10-30

EFFECT OF HBsAg DNA VACCINE ON MOUSE ORGANS

FANG Ling ，YAO Yu一Cheng2，JING Hong—Biao,XIE Qing—dong，ZHAO Shu—min2．YU Hong — yu2;HU Yi— ping;Wang Zhong-hua   

  1. 1．Department ofBiology，Gu~ngo：[Medical University，Nanning 530021，China；2．Department of Cell Biology，Second Military Medical University，Shanghai 200433，China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2000-10-30 Published:2000-10-30

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摘要： 目的：用乙型肝炎病毒(HBV．Hepatitis Bvirus)表面抗原DNA疫苗对小鼠进行基因免疫，观察主要脏器病理形态改变，探讨该DNA疫苗是否对肝、肾、脾等有损伤作用。方法：用己型肝炎表面抗原(HBsAg)DNA疫苗经肌肉注射法免疫小鼠：ELISA法检测小鼠血清抗HBaAg抗体水平。免疫l2周后取小鼠肝、肾、脾等组织，观察其病理形态改变情况。结果：DNA疫苗接种1周后．免疫组小鼠血清中可检测到抗HBsAg抗体．12周后多只小鼠脾脏明显增大，肝、肾组织切片染色观察，可见免疫组小鼠的肝、肾组织均有不同程度的病理改变。结论：HBsAg DNA疫苗可诱导小鼠机体产生特异性抗体(可诱发全面、持久的免疫应答)持续 周以上，同时伴有j卑脏肿大和肝肾损伤提示．该DNA疫苗表达产物与免疫小鼠脏器病变有一定的关系，对其临床应用前景值得进一步探索。

关键词: 乙型肝炎病毒, 表面抗原, DNA疫苗, 病理改变, 脏器

Abstract: To study the pathological change Of organs of mice immunitied byⅫ Ag DNA vaccine．Methods： HBsAg vaccine was i~ected into muscles of mice Anti—HBsAg antibody w analyzed by ELISA．After 12 weeks，the immunitied mi ce were killed and those organs were analyzed by pathological methods． Results：Anti 一Ⅻ Ag antibody in mice was positive after one wek．Spleens of mice were 4 folds larger than contro1． Kindeys and 1Nets were severe iniured．Conclusion：sAg vacine can induce mice to create antibody，lasting 12 weeks and more．But，pathology ch~rlge of organs indicated that the production of vaccine or related react_ may give rise tO the damage of the organs of mice ．This result make~to study the．safety of H&Ag vaccine further

Key words: Hepa titis B virus, surface an tigen, DNA vaccine, pathology change

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