小剂量电离辐射引起人淋巴细胞染色体的不分离

doi:10.3969/j.issn.1004-616x.2003.02.002

癌变·畸变·突变 ›› 2003, Vol. 15 ›› Issue (2): 67-71.doi: 10.3969/j.issn.1004-616x.2003.02.002

小剂量电离辐射引起人淋巴细胞染色体的不分离

李　华，刘　雯，李锦燕，左　蠧

复旦大学上海医学院细胞与遗传医学系，上海　200032

收稿日期:2002-07-03 修回日期:2003-02-14 出版日期:2003-04-30 发布日期:2003-04-30
通讯作者: 左　蠧

EFFECT OF LOW DOSE IONIZING RADIATION ON HUMAN LYMPHOCYTES CHROMOSOME NON-DISJUNCTION

LI Hua, LIU Wen, LI Jin-yan, et al.

Department of Cellular and Genetic Medicine,Shanghai Medical College,Fudan University, Shanghai 200032, China

Received:2002-07-03 Revised:2003-02-14 Online:2003-04-30 Published:2003-04-30
Contact: ZUO Ji

摘要/Abstract

摘要： 目的：探讨低剂量电离辐射引起人外周血染色体数目的变化及不分离情况。方法：用人外周血中期染色体制备并计数，用χ 2检验作统计学分析，检测在0、0.1、0.25、0.5、1.0、2.0 Gy γ-射线照射后，不同时间给予0.5 Gy γ-射线照射后及在接种前后给予不同次数0.5 Gy γ-射线照射后非整倍体率及超二倍体 / 非整倍体变化。结果：在0～2.0 Gy剂量范围内，非整倍体率随剂量增加从21.30 ％增加到55.56 ％，超二倍体 / 非整倍体从8.70 ％增加到44.00 ％；接种后照射比接种前照射非整倍体率及超二倍体 / 非整倍体高，且均比对照组高，在接种后不同时间照射的细胞无明显差别；在接种前照射，随照射次数增加到3次，非整倍体率从21.30 ％增加到47.85 ％，超二倍体 / 非整倍从8.70 ％增加到24.49 ％。结论：小剂量电离辐射对非整倍体特别是染色体不分离的影响具有剂量效应、次数效应，且接种后照射比接种前照射变化更明显。

关键词: 小剂量电离辐射, 人淋巴细胞, 非整倍体, 染色体不分离

Abstract: Purpose:To explore the changes of chromosome number and chromosome non-disjunction in human lymphocytes treated with low dose ionizing radiation. Methods: The changes of chromosome number were measured by metaphase chromosome counts, and statistic an analysed with χ 2 test. Results: After treated whole blood with 0, 0.1, 0.25, 0.5, 1.0, 2.0 Gy γ-rays, aneuploidy rate increased from 21.30 ％ to 55.56 ％ as dose increased, so did hyperdiploid / aneuploid, which increased from 8.70 ％ to 44.00 ％. After treated with 0.5 Gy at different time, the two targets were significantly higher than control(P<0.01), and the degree of increasing of the culture radiated was higher than that of the whole blood radiated. When the culture was radiated,the two targets had no difference in different radiation time. When the whole blood was radiatied, the two targets had the tendency to increase as the radiation times increased. Conclusion: The effect of low dose γ-rays on aneuploid, especially chromosome non-disjunction is dose-dependent and time-dependent. And the effect is outstanding when the culture radiated than when the whole blood was radiated.

Key words: low dose ionizing radiation, human lymphocyte, aneuploidy, chromosome non-disjunction

李　华, 刘　雯, 李锦燕, 左　蠧. 小剂量电离辐射引起人淋巴细胞染色体的不分离[J]. 癌变·畸变·突变, 2003, 15(2): 67-71.

LI Hua, LIU Wen, LI Jin-yan, et al.. EFFECT OF LOW DOSE IONIZING RADIATION ON HUMAN LYMPHOCYTES CHROMOSOME NON-DISJUNCTION[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2003, 15(2): 67-71.

[1]	倪娟,邹天宁,汪旭. 叶酸和核黄素对乳腺癌患者淋巴细胞8和17号染色体非整倍体的影响[J]. 癌变·畸变·突变, 2013, 25(1): 26-30.
[2]	马明福李练兵吕　静李新生陈晓星赵乐天. 多色荧光原位杂交检测弱精子症的非整倍体[J]. 癌变·畸变·突变, 2008, 20(3): 239-241.
[3]	黄继华, 杨芳炬. 氟哌啶醇对小鼠卵母细胞染色体的影响[J]. 癌变·畸变·突变, 2005, 17(3): 144-147.
[4]	刘新霞, 黄国贤, 邓丽霞, 郑履康. 多色荧光原位杂交方法同时检测人精子染色体数目畸变和结构畸变[J]. 癌变·畸变·突变, 2004, 16(1): 9-12.
[5]	丁银润, 王晓燕, 汪　旭. FISH技术评价昆明山海棠在小鼠骨髓细胞中的非整倍体诱发效应[J]. , 2002, 14(3): 144-146.
[6]	马国建1, 孟正木2, 王佾先1, 张琴芬1, 薛开先1 3. 无花果提取物致突变及抗突变研究①[J]. , 2002, 14(3): 177-179.
[7]	李　欣1; 郑履康2; 邓丽霞2; 张　桥2. 双色荧光原位杂交检测正常人精子9 、18 号染色体非整倍体率[J]. , 2002, 14(2): 80-083.
[8]	宋忠魁, 梁子卿, 汪　旭. 乙酰胆碱受体抑制剂对猪脑微管蛋白聚合的影响[J]. , 2001, 13(2): 70-073.
[9]	杨明杰2 　综述,曹　佳1 , 余争平2 　审校. 微核实验的分子生物学研究进展[J]. , 2000, 12(4): 237-241.
[10]	杨录军,曹　佳. 微核形态用于区分非整倍体毒剂和染色体断裂剂的研究[J]. , 2000, 12(4): 196-199.
[11]	和智君,汪　旭. d2筒箭毒碱对V79 细胞和小鼠骨髓细胞有丝分裂的影响[J]. , 2000, 12(1): 8-011.
[12]	和智君　李晓琼　汪　旭. 胆碱受体拮抗剂在小鼠骨髓细胞中的C- 有丝分裂效应研究[J]. , 1999, 11(4): 165-167.
[13]	徐德祥1 　郑履康2 　邓丽霞2. 双色荧光原位杂交检测丙烯腈接触男工精子性染色体数目畸变[J]. , 1999, 11(4): 167-171.
[14]	和智君　汪　旭. 阿托品对中国仓鼠V79 细胞有丝分裂过程的影响[J]. , 1999, 11(3): 125-127.
[15]	郑履康　邓丽霞　张　桥. 荧光原位杂交(FISH) 检测人精子染色体非整倍体率方法的研究[J]. , 1998, 10(5): 267-271.

小剂量电离辐射引起人淋巴细胞染色体的不分离

EFFECT OF LOW DOSE IONIZING RADIATION ON HUMAN LYMPHOCYTES CHROMOSOME NON-DISJUNCTION

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价