膀胱癌多药耐药基因MDR_1与金属硫蛋白_3表达的关系

doi:10.3969/j.issn.1004-616x.2007.05.019

癌变·畸变·突变 ›› 2007, Vol. 19 ›› Issue (5): 412-415.doi: 10.3969/j.issn.1004-616x.2007.05.019

膀胱癌多药耐药基因MDR_1与金属硫蛋白_3表达的关系

朱　斌/ 杨罗艳/ 赵晓昆

(中南大学湘雅二医院泌尿外科，长沙　410011)

收稿日期:2006-07-31 修回日期:2007-04-28 出版日期:2007-09-30 发布日期:2007-09-30
通讯作者: 朱　斌

Relationship Between Expressions of MT_3 and MDR_1 in Bladder Cancer

ZHU Bin, YANG Luo_yan,ZHAO Xiao_kun

(Department of Urology,Xiangya Second Hospital of Central South University, Changsha 410011,China)

Received:2006-07-31 Revised:2007-04-28 Online:2007-09-30 Published:2007-09-30
Contact: ZHU Bin

摘要/Abstract

摘要： 背景与目的：探讨膀胱移行细胞癌中多药耐药基因(MDR_1)与金属硫蛋白_3(MT_3) 的表达及其相互关系。材料与方法：采用RT_PCR 对11例正常膀胱黏膜和23例膀胱移行细胞癌(BTCC)组织MT_3和 MDR_1 mRNA表达情况进行分析。结果: 11例正常膀胱黏膜组织中MDR_1和MT_3 mRNA 表达阳性率分别为9.1％(1/11) 和0％(0/11),23例膀胱癌组织中MDR_1 和 MT_3表达阳性率分别为56.5％(13/23) 和78.3％(18/23) ；膀胱移行细胞癌组织与正常膀胱黏膜中MDR_1、MT_3表达阳性率相比，差异均有统计学意义(P<0.05)，而MDR_1与MT_3的表达无明显相关关系(P>0.05)。结论： MDR_1 可能为肿瘤组织分化程度的一个标志，MT_3的表达与膀胱移行细胞癌的早期诊断相关,可能成为一种新的肿瘤标志物。

关键词: 膀胱移行细胞癌, 金属硫蛋白, 多药耐药性

Abstract: BACKGROUND ＆ AIM: To investigate the role of MT_ 3 in transitional cell carcinomas of bladder and the possible relation with MDR_1. MATERIALS AND METHODS: Using RT_PCR technique, the expressions of MT_3 and MDR_1 mRNA in 11 specimens from normal bladder mucosa and 23 specimens from bladder transitional cell cancer(BTCC) were measured. RESULTS: MT_3 and MDR_1_positive expression rates were found in 0 and 9.1％ in normal bladder mucosa ,respectively .But they were 78.3％ and 56.5％ in 23 BTCC specimens ，respectively. The positive rate was significantly higher in BTCC specimens than in normal tissue (P<0.05) .The expressions of MT_3 and MDR_1 were not related with the grade ,stage and recurrence after postoperative intravesical chemotherapy. CONCLUSION: MT_3 mRNA could not be identified in normal bladder mucosa, but highly expressed in bladder neoplasm，MT_3 might be used as a tumor marker.

Key words: bladder neoplasms, metallothionein, multidrug resistance

中图分类号:

R737.14

朱　斌/ 杨罗艳/ 赵晓昆. 膀胱癌多药耐药基因MDR_1与金属硫蛋白_3表达的关系[J]. 癌变·畸变·突变, 2007, 19(5): 412-415.

ZHU Bin, YANG Luo_yan, ZHAO Xiao_kun. Relationship Between Expressions of MT_3 and MDR_1 in Bladder Cancer[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2007, 19(5): 412-415.

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膀胱癌多药耐药基因MDR_1与金属硫蛋白_3表达的关系

Relationship Between Expressions of MT_3 and MDR_1 in Bladder Cancer

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