白藜芦醇对结肠癌细胞生长的影响及作用机制探讨

doi:10.3969/j.issn.1004-616x.2007.06.009

癌变·畸变·突变 ›› 2007, Vol. 19 ›› Issue (6): 460-462.doi: 10.3969/j.issn.1004-616x.2007.06.009

白藜芦醇对结肠癌细胞生长的影响及作用机制探讨

崔文明1 /赵丽娟2 /杜海方1 /李　宁1

1.中国疾病预防控制中心营养与食品安全所，2.河北医科大学

收稿日期:2006-11-27 修回日期:2007-09-04 出版日期:2007-11-30 发布日期:2007-11-30

Effects and Mechanisms of Resveratrol on Human Colon Cell Line Proliferation

CUI Wen-ming1, ZHAO Li-juan2, DU Hai-fang1, LI Ning1

1.National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention,2.Heibei Medical University shijiazhuang

Received:2006-11-27 Revised:2007-09-04 Online:2007-11-30 Published:2007-11-30

摘要/Abstract

摘要： 背景与目的：探讨白藜芦醇(Rev)对结肠癌细胞系HT_29生长的影响，并结合细胞内环氧酶_2(COX_2)以及凋亡相关蛋白Bcl_2和Bax的表达情况探讨白藜芦醇的作用机制。材料与方法：分别以含有0、12.5、25、50、100 μmol/L白藜芦醇(Rev)的完全培养液培养HT_29细胞。用MTT法检测HT_29细胞生长情况，用western blot法测定细胞内COX_2的含量，用免疫细胞化学法检测细胞内凋亡相关蛋白Bcl_2和Bax的表达情况。结果：与对照组相比，Rev能够抑制HT_29细胞的生长，且呈剂量和时间依赖性。以不同浓度的Rev作用HT_29细胞48 h后发现，Rev对细胞内Bcl_2的表达水平没有明显影响，但50和100 μmol/L的Rev能够明显抑制细胞内COX_2的表达，并且100 μmol/L的Rev能够明显上调细胞内Bax的表达。结论： Rev能够抑制结肠癌细胞HT_29的增殖，且具有剂量和时间依赖性，其作用与Rev能够抑制细胞内COX_2的表达以及上调Bax的表达有关。

关键词: 白藜芦醇, 结肠癌细胞

Abstract: BACKGROUND ＆ AIM:To investigate the effects and mechanisms of resveratrol on human colon cell line HT_29. MATERIALS AND METHODS: HT_29 cells were incubated with 0,12,5,25,50,100 μmol/L resveratrol. The proliferation of HT_29 cells was measured by MTT,the COX_2 were analyzed by Western blot,the Bcl_2 and Bax were detected by immunohistochemistrical method. RESULTS:MTT results revealed that resveratrol could inhibit the proliferation of HT_29 cells in a dose and time_dependent manner.And after HT_29 cells were treated with different concentrations of resveratrol for 48 h, resveratrol at concentrations of 50 and 100 μmol/L could inhibit the expression of COX_2,and resveratrol at concentrations of 100 μmol/L could induce the expression of Bax. CONCLUSION: Resveratrol could inhibit the proliferation of HT_29 cells in a dose and time_dependent manner. The inhibition of COX_2 protein expression and the induction of Bax protein expression may be the underlying mechanisms.

Key words: resveratrol, human colon cell line

崔文明 /赵丽娟 /杜海方 /李　宁. 白藜芦醇对结肠癌细胞生长的影响及作用机制探讨[J]. 癌变·畸变·突变, 2007, 19(6): 460-462.

CUI Wen-ming, ZHAO Li-juan, DU Hai-fang, LI Ning. Effects and Mechanisms of Resveratrol on Human Colon Cell Line Proliferation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2007, 19(6): 460-462.

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白藜芦醇对结肠癌细胞生长的影响及作用机制探讨

Effects and Mechanisms of Resveratrol on Human Colon Cell Line Proliferation

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