关键词: 染色体涂染, 易位畸变, 回顾性剂量估算

Abstract: BACKGROUND AND AIM: To investigate the applicability of painting method in retrospective dose estimation. MATERIALS AND METHODS: Healthy human peripheral blood samples were irradiated with 0－5.00 Gy 60Co γ-rays. Then the chromosome translocations in these samples were detected with painting method using chromosomes 1, 2 and 4 probes. The dose-response curve of absorbed dose and genome translocation frequency were established. The retrospective doses were estimated for 3 individuals, who previously had accidental radiation exposure and had biodosimetry data shortly after the exposure. And similar estimation was done for another individual without biodosimetry data but with the physical dose data. RESULTS: The genome translocation rates induced by 0－5.00 Gy 60Co γ-rays detected by painting were increased with the absorbed dose. The dose-response curve was =0.043D2＋0.006D＋0.0036 for painting.The retrospective doses for the 4 individuals were similar with the previous biodosimetry data or the physical dose data. CONCLUSION: Dose reconstruction for previous radiation exposure individuals could be carried out with the painting method established in this study.

Key words: chromosome painting, translocation, retrospective dose estimation