鞘磷脂水解产物对结肠癌细胞中碱性鞘磷脂酶的影响

doi:10.3969/j.issn.1004-616x.2009.06.003

癌变·畸变·突变 ›› 2009, Vol. 21 ›› Issue (6): 418-421.doi: 10.3969/j.issn.1004-616x.2009.06.003

鞘磷脂水解产物对结肠癌细胞中碱性鞘磷脂酶的影响

张萍1;袁丽杰1;赵蕾1;李百祥2

1. 哈尔滨医科大学大庆校区医学检验系，黑龙江　大庆 163319； 2. 哈尔滨医科大学公共卫生学院毒理学教研室，黑龙江　哈尔滨 150081

收稿日期:2009-05-20 修回日期:2009-06-15 出版日期:2009-11-30 发布日期:2009-11-30
通讯作者: 张萍

Effect of Sphingomyelin Hydrolysates on Alkaline Sphingomyelinase Expression in Human Cancer HT-29 Cell

ZHANG Ping1; YUAN Li-jie1; ZHAO Lei1; LI Bai-xiang2

1.Department of Biological Chemistry, Daqing Campus of Harbin Medical University, Daqing 163319, Heilongjiang; 2. Department of Toxicology, Public Health College, Harbin Medical University, Harbin 150081, Heilongjiang, China

Received:2009-05-20 Revised:2009-06-15 Online:2009-11-30 Published:2009-11-30
Contact: ZHANG Ping

摘要/Abstract

摘要： 背景与目的：碱性鞘磷脂酶(alk-SMase)是鞘磷脂(SM)新陈代谢和发挥抑制结肠癌作用的关键酶。通过体外实验探讨SM的水解产物的神经酰胺(Cer)和鞘氨醇(Sph)对人结肠癌细胞HT-29中alk-SMase表达的影响。材料与方法：分别用12.5、25、50 μmol/L的C2-Cer和Sph处理HT-29细胞12、24、48 h后，分别用Western blot法和RT-PCR检测细胞中alk-SMase的蛋白水平和mRNA表达水平的变化。实验并设DMSO溶剂对照组。结果： C2-Cer和Sph作用HT-29细胞后，其alk-SMase蛋白及其mRNA的表达下调。结论： Cer和Sph对HT-29细胞中alk-SMase的表达具有负反馈调节作用。

关键词: 神经酰胺, 鞘氨醇, 碱性鞘磷脂酶, 人结肠癌HT-29细胞

Abstract: BACKGROUND AND AIM: Alkaline sphingomyelinase(alk-SMase) is the key enzyme of sphingomyelin(SM) metabolism, and an inhibitor of colon cancer. Through vitro experiments, we examined the alk-SMase expression in human colon cancer HT-29 cells, which produced the hydrolysis products of SM, ceramide(Cer) and sphingosine(Sph). MATERIALS AND METHODS: After treatment with 12.5,25 and 50 μmol/L of C2-Cer and Sph for 12,24 and 48 hours, we tested the alk-SMase mRNA and protein expressions in HT-29 cells. DMSO was the control. RESULTS: Compared with the control group, the alk-SMase expression of protein and mRNA level was reduced by C2-Cer and Sph in HT-29 cells, With a clear dose-response relationship. CONCLUSION: Cer and Sph exerted negative feedback on alk-SMase. Cer and Sph might have induced apoptosis directly in colon cancer cells, rather than through hydrolysis of SM.

Key words: ceramide, sphingosine, alkaline sphingomyelinase, colon cancer HT-29 cells

中图分类号:

R735.3＋5

张萍, 袁丽杰, 赵蕾, 李百祥. 鞘磷脂水解产物对结肠癌细胞中碱性鞘磷脂酶的影响[J]. 癌变·畸变·突变, 2009, 21(6): 418-421.

ZHANG Ping, YUAN Li-jie, ZHAO Lei, LI Bai-xiang. Effect of Sphingomyelin Hydrolysates on Alkaline Sphingomyelinase Expression in Human Cancer HT-29 Cell[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2009, 21(6): 418-421.

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[2]	白艳艳1，2/吴艳萍2/张晓峰2/李百祥2，*. ERK和SAPK/JNK通路在神经酰胺致HT-29细胞凋亡中的作用[J]. 癌变·畸变·突变, 2012, 24(6): 413-417.
[3]	张　萍(综述)/ 李百祥(审校). 碱性鞘磷脂酶与结肠癌发生发展之间的关系[J]. , 2008, 20(5): 409-411.

鞘磷脂水解产物对结肠癌细胞中碱性鞘磷脂酶的影响

Effect of Sphingomyelin Hydrolysates on Alkaline Sphingomyelinase Expression in Human Cancer HT-29 Cell

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