›› 2011, Vol. 23 ›› Issue (2): 123-127.doi: 10.3969/j.issn.1004-616x.2011.02.010

• 论著 • 上一篇    下一篇

联合检测Syk及nm23H1在大肠癌中的表达及其意义

张 钧1，孙国贵1，付占昭2，王雅棣3,﹡，徐 洪4，顾 涛2   

  1. 1.河北医科大学第四医院放疗科，河北 石家庄 050011； 2. 秦皇岛市第一医院肿瘤科，河北 秦皇岛 06600； 3. 北京军区总医院放疗科，北京 100700； 4. 河北联合大学实验中心，河北 唐山 063000
  • 收稿日期:2010-12-27 修回日期:2011-01-06 出版日期:2011-03-30 发布日期:2011-03-30
  • 通讯作者: 王雅棣

Expression and clinical　 significance of combined　 detection on Syk and nm23H1 proteins in colorectal cancer

ZHANG Jun1，SUN Guo-gui1，FU Zhan-zhao2，WANG Ya-di3,*，XU Hong4，GU Tao2   

  1. 1. Department of Radiotherapy, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei; 2. Department of Oncology, The First Hospital of Qinhuangdao, Qinhuangdao 06600, Hebei; 3. Department of Radiotherapy, The Military General Hospital of Beijing PLA, Beijing 100700; 4. Department of Medical Research Center, Hebei United University, Tangshan 063000, Hebei, China
  • Received:2010-12-27 Revised:2011-01-06 Online:2011-03-30 Published:2011-03-30
  • Contact: WANG Ya-di

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摘要： 探讨候选抑癌基因脾酪氨酸激酶(spleen tyosine kinase，Syk)及转移抑制基因(metastasis suppressor gene，nm23H1)在大肠癌组织中的表达及其生物学行为之间的关系。 方法： 采用免疫组织化学SP法，分别检测60例大肠癌组织及30例癌旁正常组织中Syk及nm23H1蛋白的表达情况；并分析其与大肠癌病理特征及生物学行为之间的关系。 结果： Syk及nm23H1蛋白在大肠癌组织中的阳性表达率分别为31.7%、53.3%，在癌旁组织中的阳性表达率分别为96.7%、93.3%，癌组织中的阳性表达率均较癌旁组织明显降低(P＜0.05)。单因素分析显示Syk及nm23H1蛋白分别与淋巴结转移、浸润深度、Dukes分期密切相关(P＜0.05)，但与患者的年龄、性别、肿瘤大小、发生部位和肿瘤分化程度无明显关系(P＞0.05)。 结论： 大肠癌组织中Syk和nm23H1蛋白表达均明显低于癌旁正常组织，可能与大肠癌的发生发展及恶性程度相关。联合检测Syk及nm23H1的表达对大肠癌的临床治疗和预后判断有一定价值。

关键词: 脾酪氨酸激酶, 转移抑制基因, 大肠癌, 免疫组织化学

Abstract: To investigate the expression of Spleen tyosine kinase (Syk) and of metastasis suppressor gene (nm23H1) and to determine the relationship between their expressions and clinico-pathological features有 and biological behavior in colorectal cancer. METHODS： Immunohistochemistry (SP) was used to detect the expressions of Syk and nm23H1 in 60 colorectal cancer tissues and normal colorectal cancer mucosa (5 cm distant to the margin of colorectal cancer and no microscopic cancer infiltration). RESULTS： The positive rates of Syk and nm23H1 proteins were 31.7%， 53.3%, respectively, in colorectal carcinoma tissues but 96.7%，93.3%, respectively, in normal tissues (P＜0.05). There was a significant difference in Syk and nm23H1 protein expression between lymph node metastasis， depths of invasion and Dukes stage(P＜0.05)，but not with sex， age，tumor size，lesion site and differentiation degree(P＞0.05). CONCLUSION： The expressions of Syk and nm23H1 proteins were lower in colorectal cancer tissues， suggesting that the Syk and nm23H1 genes may be closely associated with oncogenesis and malignant degree. Detection of Syk and nm23H1 may be used for early diagnosis， clinical therapy and prognosis of colorectal carcinoma.

Key words: Syk, nm23H1, colorectal cancer, immunohistochemistry

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