›› 2011, Vol. 23 ›› Issue (5): 384-387.doi: 10.3969/j.issn.1004-616x.2011.05.015

• 检测研究 • 上一篇    下一篇

重组人乳铁蛋白的遗传毒性研究

刘 珊/冯晓莲/李晨汐/耿桂英/刘海波/支 媛/徐海滨*   

  1. 中国疾病预防控制中心营养与食品安全所，北京 100021
  • 收稿日期:2011-03-09 修回日期:2011-05-19 出版日期:2011-09-30 发布日期:2011-09-30
  • 通讯作者: 徐海滨

Genetic toxicity of recombinant human lactoferrin

LIU Shan，FENG Xiao-lian，LI Chen-xi，GENG Gui-ying，LIU Hai-bo，ZHI Yuan，XU Hai-bin*   

  1. Institute for Nutrition and Food Safety, China Center for Disease Control and Prevention, Beijing 100021, China
  • Received:2011-03-09 Revised:2011-05-19 Online:2011-09-30 Published:2011-09-30
  • Contact: XU Hai-bin

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摘要： 目的： 评价重组人乳铁蛋白的遗传毒性。方法：以重组人乳铁蛋白为受试物，按每皿62、185、556、1 667、5 000 μg 5个剂量进行Ames试验，按1.88、3.75和7.50 g/kg进行小鼠骨髓嗜多染红细胞 (PCE)微核试验和精子畸形试验，并设立对照组。结果：重组人乳铁蛋白各剂量组回变菌落数均未超过溶剂对照菌落数的2倍，亦无剂量－反应关系；各剂量组PCE百分比均未少于阴性对照组的20%，微核发生率与阴性对照组比较差异均无统计学意义 (P均>0.05)；各剂量组小鼠精子畸形发生率均显著低于阴性对照组 (P<0.05)。结论：在本实验条件下，重组人乳铁蛋白未见遗传毒性。

关键词: 遗传毒性, 重组蛋白, 人乳铁蛋白

Abstract: OBJECTIVE: To evaluate genetic toxicity of recombinant human lactoferrin (rhLf) expressed in milk from genetically modified cow. METHODS：In Ames test，TA97，TA98，TA100，and TA102 strains were treated with 62，185，556，1 667 and 5 000 μg recombinant human lactoferrin (rhLf) per plate. In mice bone marrow cell micronucleus test and mice sperm abnormality test，1.88，3.75 and 7.50 g/kg rhLf groups and control groups were set. RESULTS：Back mutation colonies of rhLf groups did not exceed more than twice that of the control in Ames test and there was no dose-response relationship. In mice bone marrow cell micronucleus test，no significant difference was found in PCE/RBC and micronucleus rate between rhLf-treated groups and negative control group. In mice sperm abnormality test，sperm abnormality rate of rhLf-treated groups was lower than that of negative control. CONCLUSION：No genetic toxicity was observed in recombinant human lactoferrin in this study.

Key words: genetic toxicity, recombinant protein, human lactoferrin

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