MARDHRS4的鉴定及其对DHRS4转录的调节

doi:10.3969/j.issn.1004-616x.2013.02.009

癌变·畸变·突变

MARDHRS4的鉴定及其对DHRS4转录的调节

常晓兰¹,冯雷¹,冯广友^2，*

1. 汕头大学医学院细胞生物学教研室，广东汕头 515041；2. 汕头大学医学院病理生理学教研室，广东汕头 515041

收稿日期:2012-10-10 修回日期:2013-01-07 出版日期:2013-03-30 发布日期:2013-03-30
通讯作者: 冯广友，E-mail：gyfeng@stu.edu.cn
作者简介:常晓兰 (1964- )，女，河南省商丘市人，实验师，研究方向：细胞生物学。

The identification of MARDHRS4 and its regulation on DHRS4 transcription

CHANG Xiao-lan¹，FENG Lei¹，FENG Guang-you^2，*

1. Department of Cell Biology, Shantou University Medical College, Shantou 515041; 2. Department of Pathophysiology, Shantou University Medical College, Shantou 515041, Guangdong, China

Received:2012-10-10 Revised:2013-01-07 Online:2013-03-30 Published:2013-03-30
Contact: FENG Guang-you，E-mail：gyfeng@stu.edu.cn

摘要/Abstract

摘要：

目的：筛选新的小鼠DHRS4反义链转录本并对其功能进行研究。方法：采用快速cDNA末端扩增方法，从小鼠正常肝细胞株NCTC1469中克隆DHRS4反义链转录本，据此转录本设计合适siRNA，然后转染NCTC1469细胞，转染成功36 h后应用RT-qPCR检测细胞内DHRS4 mRNA水平的改变。结果：成功克隆出1个新的天然反义RNA，全长835 nt，属于长链非编码RNA (long non-coding RNA，LncRNA)，命名为MARDHRS4，应用siRNA干扰MARDHRS4后，DHRS4 mRNA降低约40%。结论：来自DHRS4反义链的LncRNA MARDHRS4具有增强DHRS4转录的作用。

关键词: MARDHRS4, DHRS4, 长链非编码RNA, 转录

Abstract:

OBJECTIVE: To screen the novel antisense transcriptions of DHRS4 and study their functions. METHODS：RACE was used to clone the antisense transcription of DHRS4 from the NCTC1469 cells (normal mouse liver cell line). Based on the antisense transcription cloned above，the suitable siRNA was designed and transfected the NCTC1469 cells. 36 h after successful transfection，the relative amount of DHRS4 mRNA in transfected cells was tested by RT-qPCR. RESULTS：A novel 835 nt natural antisense RNA was successfully cloned and identified. It belonged to a kind of LncRNA and was named MARDHRS4 (mouse antisense RNA of DHRS4). DHRS4 mRNA decreased about 40% after interruption of siRNA to MARDHRS. CONCLUSION：MARDHRS4，one kind of LncRNA transcripted from the opposite strand of DHRS4，may play a role in enhancing transcription of DHRS4.

Key words: MARDHRS4, DHRS4, long non-coding RNA, transcription

常晓兰,冯雷,冯广友. MARDHRS4的鉴定及其对DHRS4转录的调节[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2013.02.009.

CHANG Xiao-lan，FENG Lei，FENG Guang-you. The identification of MARDHRS4 and its regulation on DHRS4 transcription[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.02.009.

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MARDHRS4的鉴定及其对DHRS4转录的调节

The identification of MARDHRS4 and its regulation on DHRS4 transcription

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