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红花提取物不同组分的体外抑瘤实验研究

 莉,陆 雪,赵 骅,刘青杰,刘建香,封江彬*   

  1. ( 中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,北京 100088 )
  • 收稿日期:2013-04-27 修回日期:2013-08-07 出版日期:2013-09-30 发布日期:2013-09-30
  • 通讯作者: 封江彬,E-mail:msfl@sina.com
  • 作者简介: 范 莉 (1977- ),女,安徽省人,博士,副研究员,研究方向: 抗辐射药物及抗肿瘤药物研究。E-mail:fanliyhx@gmail.com
  • 基金资助:

    卫生行业科研专项 (201002009);中国疾病预防控制中心辐射防护与核安全医学所青年科研基金 (2012A002)

Anti-tumor activity of different effective fractions of safflower extract in vitro

FAN Li,LU Xue,ZHAO Hua,LIU Qing-jie,LIU Jian-xiang,FENG Jiang-bin*   

  1. (National Institute for Radiological Protection, Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention, Beijing 100088, China)
  • Received:2013-04-27 Revised:2013-08-07 Online:2013-09-30 Published:2013-09-30
  • Contact: FENG Jiang-bin,E-mail:msfl@sina.com

摘要:

目的: 探究红花提取物抑瘤作用的有效组分。方法:选择人慢性粒细胞白血病K562细胞、人肝癌SMMC7721细胞、人宫颈癌HeLa细胞、人肺癌细胞H1299及A549共5株人常见肿瘤细胞株,用细胞计数试剂盒 (cell counting kit-8,CCK-8)方法对红花提取物及大孔树脂水-乙醇洗脱后的不同有效组分共6个供试品进行体外抑瘤作用评价,绘制浓度效应曲线,计算半数抑制浓度(IC50),比较各有效组分的抑瘤效果。结果:红花提取物各有效组分对肺癌细胞的抑制效果较好,IC50为16.24~189.86 μg/mL,其次为K562、SMMC7721及HeLa细胞。红花提取物脂溶性组分抑瘤作用较水溶性组分好,如红花提取物95%醇溶组分对5株肿瘤细胞均有明显的抑制作用,IC50为16.24~66.51 μg/mL。结论:红花提取物在体外对多种癌细胞有抑制作用,尤其是对肺癌细胞,其抑瘤作用有效物质可能是脂溶性组分。

关键词: 红花, 体外抑瘤实验, 半数抑制浓度, CCK-8

Abstract:

 OBJECTIVE: To find anti-tumor effective fractions of Carthamus tinctorius,and evaluate the cytotoxic activity of its different effective fractions observed. METHODS:Cytotoxicity of six samples of safflower extract isolated by macroporous resin were evaluated in vitro against K562 (human leukemia cancer),SMMC7721 (human liver cancer), HeLa (human cervical cancer),H1299 and A549 (human lung cancer) by CCK-8 assay. Concentration-effect curves and IC50 values were calculated. RESULTS:Safflower samples showed better cytotoxicity against H1299 and A549 with IC50 values of 16.24 to 189.86 μg/mL,and moderate cytotoxic activity or no activity against K562,SMMC7721 and HeLa. Insoluble fractions of safflower extract had better cytotoxic activity than water-soluble fractions. 95% alcohol elution fraction of the safflower extract had obvious cytotoxicity against five kinds of tumor cell lines with IC50 values of 16.24 to 66.51 μg/mL. CONCLUSION:Safflower extract could inhibit a variety of cancer cells,especially human lung cancer cell. The effective fraction may be the fat soluble components.

Key words: Carthamus tinctorius L., inhibiting tumor assay in vitro, IC50;CCK-8