关键词: 食管癌细胞, hsa-miR-21-3p, 侵袭, 迁移

Abstract:

 OBJECTIVE: To explore the potential role of hsa-miR-21-3p in invasion and migration ability of esophageal carcinoma cell line Eca9706. METHODS：Quantitative real-time PCR was used to evaluate the expression level of miR-21-3p in Eca9706 cells and control transfected with 30 nmol/L of hsa-miR-21-3p mimics after 48 h. Transwell and wound healing experiment were carried out to determine cell invasion and migration ability of Eca9706 cells transfected with hsa-miR-21-3p mimics. RESULTS：Hsa-miR-21-3p expression level in hsa-miR-21-3p mimics- transfected Eca9706 was increased 1.1×104 times compared with negative control. Transwell assay showed the number of transmembrane cells in experimental group was increased 81.1% compared with negative control，suggested that over-expression of hsa-miR-21-3p enhanced the invasion ability of Eca9706 cells. Wound healing experiment demonstrated the capacity of Eca9706 cells migration was strengthened after transfection with hsa-miR-21-3p mimics. CONCLUSION：Up-regulation of hsa-miR-21-3p might serve as an oncogene and participate in the invasion and metastasis of esophageal cancer.