大鼠脂肪间充质干细胞移植治疗急性放射病的安全性

doi:10.3969/j.issn.1004-616x.2013.06.008

癌变·畸变·突变

大鼠脂肪间充质干细胞移植治疗急性放射病的安全性

许文黎,张伟,秦秀军,岳娟,李曙芳,王永丽,闻建华*

( 中国辐射防护研究院药物安全评价中心，山西太原 030006 )

收稿日期:2013-09-16 修回日期:2013-10-20 出版日期:2013-11-30 发布日期:2013-11-30
通讯作者: 闻建华，E-mail：jianhua570621@163.com
作者简介:许文黎 (1986- )，女，河南省南阳市人，硕士，研究实习员，研究方向：放射毒理学。E-mail：xwl1986714@163.com

Safety of rat adipose-derived mesenchymal stem cell transplantation in the treatment of acute radiation sickness

XU Wen-li，ZHANG Wei，QIN Xiu-jun，YUE Juan，LI Shu-fang，WANG Yong-li，WEN Jian-hua*

(China Institute for Radiation Protection, Taiyuan 030006, Shanxi, China)

Received:2013-09-16 Revised:2013-10-20 Online:2013-11-30 Published:2013-11-30
Contact: WEN Jian-hua，E-mail：jianhua570621@163.com

摘要/Abstract

摘要：

目的： 探讨急性放射性大鼠经静脉输注异体脂肪间充质干细胞 (adipose-derived mesenchymal stem cells，ADSCs)后的安全性。方法：SPF级雄性SD大鼠45只，随机分为空白对照组，照射对照组和照射移植组，每组15只。体外分离培养大鼠脂肪间充质干细胞，照射对照组与照射移植组大鼠经60Co治疗机一次性7 Gy剂量全身照射后，照射移植组大鼠按10 mL/kg尾静脉注射脂肪间充质干细胞，细胞浓度为1×106/mL；空白对照组和照射对照组注射等体积的无菌生理盐水。分别于照射后16和34 d进行血液学、血液生化学和组织病理学检查。结果：移植组大鼠体质量、血常规、血液生化与照射对照组相比差异无统计学意义(P>0.05)；移植组大鼠脏器病理学观察与照射组、空白对照组相比无明显差别；未见移植组大鼠出现急慢性移植物抗宿主病反应。结论：急性放射性大鼠异体静脉输注脂肪间充质干细胞是安全可行的，对受者无不良影响。

关键词: 脂肪间充质干细胞, 急性放射病, 干细胞移植, 安全性

Abstract:

OBJECTIVE: To investigate the safety of intravenous injection of allogeneic adipose-derived mesenchymal stem cells (ADSCs) in rats after acute radiation. METHODS：A total of 45 SPF male SD rats were equally and randomly divided into three groups：blank control group，radiation control group and radiation grafting group. Rat ADSCs were isolated and cultured in vitro. Irradiated control group and irradiated grafting rats were received 7 Gy dose total body irradiation once. Rats in each group received injection via tail vein，with 10 mL/ kg of 1×106 ADSCs as the irradiated grafting group and the equal volume physiological saline as blank control group and irradiated control group. The rats were observed for 34 days for their general reactions before they were sacrificed. Blood samples were collected for hematological studies at the 16th day and biochemical studies at the 34th day. Visceral organs were inspected and studied pathologically. RESULTS：No significant difference in weight，hematological and biochemical studies was detected between radiation grafting and radiation control groups(P<0.05). No significant difference was found in histopathological examination in the visceral organs among blank control，radiation control and radiation grafting groups，no appearance of acute and chronic graft versus host disease was obsessed. CONCLUSION：Intravenous infusion of allogeneic mesenchymal stem cells was feasible and safe. No side effects were found in recipients.

Key words: adipose-derived mesenchymal stem cells, acute radiation sickness, stem cell transplantation, security

许文黎,张伟,秦秀军,岳娟,李曙芳,王永丽,闻建华. 大鼠脂肪间充质干细胞移植治疗急性放射病的安全性[J]. 癌变·畸变·突变, doi: 10.3969/j.issn.1004-616x.2013.06.008.

XU Wen-li，ZHANG Wei，QIN Xiu-jun，YUE Juan，LI Shu-fang，WANG Yong-li，WEN Jian-hua. Safety of rat adipose-derived mesenchymal stem cell transplantation in the treatment of acute radiation sickness[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.06.008.

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大鼠脂肪间充质干细胞移植治疗急性放射病的安全性

Safety of rat adipose-derived mesenchymal stem cell transplantation in the treatment of acute radiation sickness

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