癌变·畸变·突变 ›› 2016, Vol. 28 ›› Issue (1): 19-22.doi: 10.3969/j.issn.1004-616x.2016.01.004

• 论著 • 上一篇    下一篇

磷化铟/硫化锌量子点对小鼠腹腔巨噬细胞功能的影响

郑至嘉1,2, 田靖琳1,2, 梅树江3, 林桂淼2, 王晓梅2, 朱雪丹2, 唐杰4, 张可4, 陈献雄2   

  1. 1. 深圳大学生命与海洋科学学院, 广东 深圳 518060;
    2. 深圳大学医学院泌尿生殖研究所, 深圳市合成生物学工程实验室, 深圳市肿瘤转化医学重点实验室, 广东 深圳 518060;
    3. 深圳市疾病预防控制中心, 广东 深圳 518055;
    4. 深圳市罗湖区人民医院, 广东 深圳 518000
  • 收稿日期:2015-09-15 修回日期:2015-11-26 出版日期:2016-01-31 发布日期:2016-01-31
  • 通讯作者: 陈献雄,E-mail:gzcxx@szu.edu.cn E-mail:gzcxx@szu.edu.cn
  • 作者简介:郑至嘉,E-mail:2130180405@email.szu.edu.cn
  • 基金资助:
    国家自然科学基金项目(81301318);深圳市科技研发资金国家和省计划配套项目(GJHS20120628150900144);深圳市基础研究项目(JCYJ20140418182819164,JCYJ2014041809141356,JCYJ20 13 0402150311058)

Effects of InP/ZnS quantum dots on function of mouse macrophage cells

ZHENG Zhijia1,2, TIAN Jinglin1,2, MEI Shujiang3, LIN Guimiao2, WANG Xiaomei2, ZHU Xuedan2, TANG Jie4, ZHANG Ke4, CHEN Xianxiong2   

  1. 1. College of Life and Marine Science, Shenzhen University, Shenzhen 518060;
    2. The Institute of Urinary and Reproductive, the Engineering Lab of Synthetic Biology, Shenzhen Key Lab of Translational Medicine of Tumor, Shenzhen University, Shenzhen 518060;
    3. Shenzhen Center for Disease Control and Prevention, Shenzhen 518055;
    4. The People's Hospital of Luohu District, Shenzhen 518000, Guangdong, China
  • Received:2015-09-15 Revised:2015-11-26 Online:2016-01-31 Published:2016-01-31

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摘要： 目的: 通过建立体外模型研究磷化铟/硫化锌(InP/ZnS)量子点对巨噬细胞功能的影响。方法: 以不同浓度的InP/ZnS量子点作用于小鼠腹腔巨噬细胞RAW264.7不同时间后,采用荧光显微镜观察巨噬细胞对量子点的摄取情况;用CCK-8法检测量子点对巨噬细胞增殖能力的影响;用酶联免疫(ELISA)法检测量子点及免疫原CpG寡脱氧核苷酸(CpG-ODN)对肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)分泌的影响。结果: 与阴性对照组比较,2.5、5和10 μg/mL InP/ZnS量子点处理4 h后可被巨噬细胞摄取并定位于细胞浆内;当InP/ZnS量子点浓度达到5 μg/mL以上时,作用24和48 h后细胞增殖能力随量子点浓度增加而明显下降(P<0.05和P<0.01);经量子点处理的巨噬细胞在有或无CpG-ODN的刺激下,TNF-α的分泌出现不同程度增加(P<0.01),而IL-6变化不明显(P>0.05)。结论: InP/ZnS量子点可以被巨噬细胞摄取,抑制巨噬细胞的增殖能力,并促进TNF-α的分泌。

关键词: 磷化铟/硫化锌, 量子点, 巨噬细胞, 细胞毒性, 细胞因子

Abstract: OBJECTIVE: To investigate the effects of InP/ZnS quantum dots (InP/ZnS QDs) on function of macrophages using in vitro cell model. METHODS: The mouse macrophage RAW264.7 cells were exposed to different concentrations of InP/ZnS QDs. The uptake of InP/ZnS QDs by macrophages was observed under a fluorescence microscope 4 hours later. Effect of InP/ZnS QDs on cell proliferation was assessed by CCK-8 assay,and the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) released by macrophages were determined by ELISA assay. RESULTS: InP/ZnS QDs entered the cells and remained mostly in the cytoplasm in 4 hours. Cells exposed to InP/ZnS QDs showed significantly decreased cell reproduction 24 hours and 48 hours later(P<0.05,P<0.01),and the levels of TNF-α released with or without CpG-ODN stimulationby macrophages significantly increased 24 hours later(P<0.01),but the levels of IL-6 remained unchanged. In addition,InP/ZnS QDs exposure led to increased release of TNF-α by macrophages following re-stimulation with CpG-ODN. CONCLUSION: The results suggest that InP/ZnS QDs could be taken up by macrophages,and could inhibit the cell viability and increase release of TNF-α.

Key words: InP/ZnS, quantum dots, macrophages, cytotoxicity, cytokine

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