应用时差成像培养系统观察早期胚胎的卵裂模式对其发育潜能的影响

doi:10.3969/j.issn.1004-616x.2023.02.007

摘要/Abstract

摘要： 目的：通过时差成像系统(TLI)观察早期胚胎的卵裂模式，探讨卵裂模式对早期胚胎发育潜能的影响。方法：回顾性分析2019年1月—2021年7月在中山市博爱医院生殖中心行辅助生殖助孕时通过时差成像系统进行囊胚培养的1 318个胚胎的发育情况。依据双原核(2PN)受精卵的卵裂模式分为正常卵裂和异常卵裂，其中异常卵裂包括首次卵裂异常和非首次卵裂异常。分析不同卵裂模式组早期胚胎第3天(D3)的优质胚胎率、囊胚形成率、优质囊胚率及妊娠率、流产率等妊娠结局。结果：正常卵裂组和异常卵裂组的D3优质胚胎率分别为57.36%和39.47%、囊胚形成率分别为66.33%和36.62%、优质囊胚率分别为60.14%和27.61%，正常卵裂组均显著高于异常卵裂组(P<0.01)；正常卵裂组与异常卵裂组间临床妊娠率和流产率差异无统计学意义(P>0.05)。在异常卵裂组中，首次卵裂异常组的D3优质胚胎率和优质囊胚率分别为34.45%和24.64%，均显著低于非首次卵裂异常组的D3优质胚胎率58.02%和优质囊胚率(37.66%)，差异均有统计学意义(P<0.05)，在2个异常卵裂组间临床妊娠率和流产率差异无统计学意义(P>0.05)。结论：胚胎的异常卵裂影响早期胚胎的发育潜能，异常卵裂发生的时间越早对胚胎发育影响越大。

关键词: 时差成像系统, 早期胚胎, 卵裂模式, 胚胎发育, 辅助生殖技术

Abstract: OBJECTIVE：To observe cleavage patterns of embryos in early stages by time-lapse imaging system，and to evaluate effects of the cleavage patterns on developmental potential of embryos in early stage. METHODS：From January 2019 to July 2021，development of 1 318 embryos cultured with blastocysts which were recorded by the time-lapse imaging system in the reproductive center were retrospectively analyzed. According to the cleavage mode of two prokaryotic (2PN) fertilized eggs，they were divided into normal and abnormal cleavages. Abnormal cleavages included first cleavage anomaly and non-first cleavage anomaly. Pregnancy outcomes such as rates of high-quality embryo， blastocyst formation， high-quality blastocyst， pregnancy， and abortion of early embryos in different cleavage pattern groups were analyzed on day 3. RESULTS： The D3 high-quality embryo rates were 57.36% and 39.47% respectively； blastocyst formation rates were 66.33% and 36.62% respectively； high-quality blastocyst rates were 60.14% and 27.61% respectively in the normal cleavage group and in the abnormal cleavage group. These were significantly higher than those in the abnormal cleavage group (P<0.01). There was no significant difference in clinical pregnancy and miscarriage rates between the normal cleavage and the abnormal cleavage groups(P>0.05). In the abnormal cleavage group，the rates for D3 high-quality embryos and high-quality blastocysts were 34.45% and 24.64% in the first abnormal cleavage group and they were significantly lower than those in the non-first abnormal cleavage group of 58.02% and 37.66% (P<0.05). There was no significant difference in the rates of clinical pregnancy and abortion between the first cleavage anomaly group and the non-first cleavage anomaly group. CONCLUSION：Abnormal cleavage of embryos reduced developmental potential of early embryo. The earlier the abnormal cleavage occurred，the greater the impact on embryonic development.

Key words: time-lapse imaging system, early embryo, cleavage pattern, embryonic development, assisted reproductive technology

中图分类号:

Q132.4

许小琴, 程立子, 罗鑫, 于文娟, 霍骏业, 刘树沅, 林秀峰. 应用时差成像培养系统观察早期胚胎的卵裂模式对其发育潜能的影响[J]. 癌变·畸变·突变, 2023, 35(2): 121-124,138.

XU Xiaoqin, CHENG Lizi, LUO Xin, YU Wenjuan, HUO Junye, LIU Shuyuan, LIN Xiufeng. Observation of the effects of early embryo cleavage patterns on its developmental potential using time-lapse imaging system[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(2): 121-124,138.

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