上皮性卵巢癌组织中FoxM1的表达及其临床意义

doi:10.3969/j.issn.1004-616x.2023.04.005

摘要/Abstract

摘要： 目的：探讨叉头框转录因子M1(FoxM1)在上皮性卵巢癌组织中的表达及其与患者临床病理指标的关系，并研究其与上皮-间质转化(EMT)相关因子E-钙黏蛋白(E-cadherin)和波形蛋白(vimentin)的相关性。方法：采用免疫组织化学法检测41例上皮性卵巢癌、17例卵巢交界性肿瘤和20例正常卵巢组织中FoxM1、E-cadherin和vimentin蛋白的表达情况。采用卡方检验比较上皮性卵巢癌组织和正常卵巢组织中FoxM1蛋白的表达差异，分析FoxM1、E-cadherin和vimentin蛋白表达与上皮性卵巢癌患者临床病理指标的相关性及3种蛋白表达水平的相关性。结果：上皮性卵巢癌组织中FoxM1、E-cadherin和vimentin阳性表达率分别为95.12%、82.93%和70.73%，高表达率分别为51.22%、53.66%和46.34%。上皮性卵巢癌组织中FoxM1阳性表达率和高表达率均显著高于卵巢交界性肿瘤和正常卵巢组织(P<0.05)。上皮性卵巢癌组织中FoxM1、E-cadherin和vimentin表达情况与患者临床分期、有无淋巴结转移有关，临床分期III~IV期、有淋巴结转移的患者卵巢癌组织中FoxM1、vimentin高表达率分别高于I~II期、无淋巴结转移患者，相关E-cadherin高表达率则显著低于I~II期、无淋巴结转移患者(P<0.05)。浆液性癌组织中vimentin高表达率显著高于透明细胞癌(P<0.05)。Spearman相关分析结果表明，上皮性卵巢癌组织中FoxM1、vimentin表达水平间存在正相关(φ=0.562，P<0.05)，且二者与E-cadherin表达水平间均呈负相关(φ值分别为-0.440和-0.366，均为P<0.05)。结论：FoxM1蛋白在上皮性卵巢癌组织中表达明显上调，与患者临床分期、淋巴结转移相关，FoxM1促进上皮性卵巢癌发生、进展和转移可能与其促进了EMT过程有关。

关键词: 上皮性卵巢癌, 叉头框转录因子M1, 上皮-间质转化, E-钙黏蛋白, 波形蛋白, 病理指标

Abstract: OBJECTIVE： To investigate expression of forkhead box protein M1 (FoxM1) in epithelial ovarian cancers and to determine its association with clinicopathological characteristics，and with epithelial mesenchymal transition (EMT)-related factors，E-cadherin and vimentin. METHODS： Expressions of FoxM1，E-cadherin and vimentin in 41 cases of epithelial ovarian cancer，17 cases of borderline ovarian tumors and 20 cases of normal ovarian tissues were detected by immunohistochemistry. χ² test was used to compare differences in the expression of FoxM1 between epithelial ovarian cancer tissues，borderline ovarian tumors and normal ovarian tissues. In addition，correlations were investigated among expressions of FoxM1，E-cadherin and vimentin，and clinicopathological characteristics of epithelial ovarian cancer patients. RESULTS： The positive expression rates of FoxM1，E-cadherin and vimentin in ovarian cancer tissues were 95.12%，82.93% and 70.73% respectively，and the high expression rates were 51.22%，53.66% and 46.34% respectively. The positive expression and high expression rates of FoxM1 in ovarian cancer tissues were significantly higher than those in the borderline ovarian cancer tissues and normal ovarian tissues (P<0.05). The expressions of FoxM1，E-cadherin and vimentin in ovarian cancer tissues were related to the clinical stages and lymph node metastasis of patients. The high expression rates of FoxM1 and vimentin in ovarian cancer tissues of patients with clinical stage III-IV and lymph node metastasis were significantly higher than those of patients with stage I-II and with no lymph node metastasis，respectively. The high expression rates of E-cadherin were significantly lower than those of patients with stage I-II and no lymph node metastasis (P<0.05). Spearman analysis showed that there were significant and positive correlations between FoxM1 and vimentin expression levels in epithelial ovarian cancer tissues(φ=0.562，P<0.05)，but there were significant and negative correlations between them and E-cadherin expression levels (φ=-0.440，-0.366，P<0.05). CONCLUSION： Expression of FoxM1 was significantly up regulated in epithelial ovarian cancers，and was related to clinical stages and lymph node metastasis. FoxM1 may therefore promote the occurrence，progression and metastasis of epithelial ovarian cancer via the EMT process.

Key words: epithelial ovarian cancer, fork head box transcription factor M1, epithelial mesenchymal transformation, E-cadherin, vimentin, pathological characteristics

中图分类号:

R737.31

覃熙媛, 朱莹, 李伟滔, 郑晓玲, 梁远秋. 上皮性卵巢癌组织中FoxM1的表达及其临床意义[J]. 癌变·畸变·突变, 2023, 35(4): 273-278.

QIN Xiyuan, ZHU Ying, LI Weitao, ZHENG Xiaoling, LIANG Yuanqiu. Expression and clinical significance of FoxM1 in epithelial ovarian cancers[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(4): 273-278.

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