油莎豆对大鼠的致畸作用研究

doi:10.3969/j.issn.1004-616x.2023.04.011

癌变·畸变·突变 ›› 2023, Vol. 35 ›› Issue (4): 306-309,315.doi: 10.3969/j.issn.1004-616x.2023.04.011

油莎豆对大鼠的致畸作用研究

李春鑫¹, 于美琴¹, 杨海龙^1,2, 宋万献¹, 张戈¹, 王会伟¹

1. 河南省农业科学院经济作物研究所, 河南郑州 450002;
2. 郑州市农林科学研究所, 河南郑州 450005

收稿日期:2023-04-21 修回日期:2023-06-25 出版日期:2023-07-30 发布日期:2023-08-04
通讯作者: 王会伟
作者简介:李春鑫，E-mail：licx2006@163.com。
基金资助:
河南省重大科技专项(211100110100)；河南省农业科学院新兴学科发展专项(2023XK01)

Teratogenicity assessment of Cyperus esculentus on rats

LI Chunxin¹, YU Meiqin¹, YANG Hailong^1,2, SONG Wanxian¹, ZHANG Ge¹, WANG Huiwei¹

1. Industrial Crops Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002;
2. Zhengzhou Institute of Agriculture and Forestry Sciences, Zhengzhou 450005, Henan, China

Received:2023-04-21 Revised:2023-06-25 Online:2023-07-30 Published:2023-08-04

摘要/Abstract

摘要： 目的：评估油莎豆对SD大鼠是否存在致畸作用。方法：以100只3月龄SPF级SD大鼠为试验动物，设置0(对照)、2、4、8 g/kg共4个油莎豆浓度，在SD大鼠孕期的第6~15天，灌胃给予受试物，每天1次。分别在受孕后第0、6、9、12、15、20天称量大鼠，于妊娠第20天采用CO₂麻醉法处死孕鼠，剖腹取出子宫，称取子宫连胎质量，记录并检查黄体数、着床数、吸收胎、早死胎、晚死胎及活胎数，活胎鼠记录胎仔体质量、体长，并检查胎鼠外观有无畸形。结果：各剂量组孕鼠在观察期内精神状态、一般活动未发现异常体征，第20天处死后大体解剖肉眼均未见明显异常；各分组试验数据统计结果显示，油莎豆对孕鼠一般情况、体质量及其增量、胚胎发育、胎鼠外观畸形、胎鼠骨骼发育、骨骼畸胎率、胎鼠内脏发育、畸胎率、孕鼠总畸胎率均无显著影响(P>0.05)。结论：在本实验条件下，油莎豆对SD大鼠无致畸作用。

关键词: 油莎豆, 致畸性, SD大鼠, 畸胎率

Abstract: OBJECTIVE： To assess whether there was a teratogenic risk from Cyperus esculentus on SPF grade pregnant SD rats. METHODS： For this study，100 3-month-old SPF grade and pregnant rats were used. They were weighed on the 0^th，6^th，9^th，12^th，15^th，and 20^th days of gestation. On the 6^th to 15^th days of gestation，rats were fed at four concentration levels of Cyperus esculentus：0 (CK)，2，4，and 8 g/kg body weight. The pregnant rats were sacrificed using CO₂ anesthesia on the 20th day of gestation. The uterus was taken out by laparotomy，and the weights of the uteri and fetuses were measured. The numbers of corpora lutea，implantation，absorption，early death，late death，and live fetus were recorded and examined. The fetal weights and body lengths were recorded for live fetal rats，and they were examined for abnormalities. RESULTS： For the pregnant rats，no abnormal physical signs were found，their general activities were normal，and no significant abnormalities were found in gross anatomy at the 20^th day of execution. Results from analysis of the data show that Cyperus esculentus had no effect on the general situation，body weight and weight gain，embryonic development，fetal mouse appearance abnormalities，fetal mouse bone development，skeletal teratogenesis rate，fetal mouse visceral development，teratogenesis rate，and total teratogenesis rate of pregnant rats. CONCLUSION： Under our experimental conditions，there was no difference in abnormalities among the different exposure dose groups and from the controls. Thus，it can be determined that Cyperus esculentus did not cause teratogenic risk under our experimental conditions.

Key words: Cyperus esculentus, teratogenicity, SD pregnant rat, teratogenesis rate

中图分类号:

R994.4

李春鑫, 于美琴, 杨海龙, 宋万献, 张戈, 王会伟. 油莎豆对大鼠的致畸作用研究[J]. 癌变·畸变·突变, 2023, 35(4): 306-309,315.

LI Chunxin, YU Meiqin, YANG Hailong, SONG Wanxian, ZHANG Ge, WANG Huiwei. Teratogenicity assessment of Cyperus esculentus on rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(4): 306-309,315.

参考文献

[1] 阳振乐. 油莎豆的特性及其研究进展[J]. 北方园艺, 2017(17): 192-201.
[2] 郭婷婷, 万楚筠, 黄凤洪, 等. 油莎豆主要营养成分及生理功能研究进展[J]. 中国油料作物学报, 2021, 43(6): 1174-1180.
[3] YU Y L, LU X Y, ZHANG T H, et al. Tiger nut (Cyperus esculentus L.): nutrition, processing, function and applications[J]. Foods, 2022, 11(4): 601.
[4] ZHANG S G, LI P Z, WEI Z M, et al. Cyperus (Cyperus esculentus L.): a review of its compositions, medical efficacy, antibacterial activity and allelopathic potentials[J]. Plants, 2022, 11(9): 1127.
[5] AYENI A O. Hoop house and field evaluation of tigernut (Cyperus esculentus L. var. sativus boeck) selections in new jersey, USA[J]. Plants, 2022, 11(7): 897.
[6] PELEGRíN C J, RAMOS M, JIMéNEZ A, et al. Chemical composition and bioactive antioxidants obtained by microwave-assisted extraction of Cyperus esculentus L. by-products: a valorization approach[J]. Front Nutr, 2022, 9: 944830.
[7] DEFELICE M S. Yellow nutsedge Cyperus esculentus L.—snack food of the Gods1[J]. Weed Technol, 2002, 16(4): 901-907.
[8] ARAFAT S M, GAAFAR A M, BASUNY A M, et al. Chufa tubers (Cyperus esculentus L.) As a new source of food[J]. World Appl Sci J, 2015, 7: 151-156.
[9] ALLOUH M Z, DARADKA H M, ABU GHAIDA J H. Influence of Cyperus esculentus tubers (tiger nut) on male rat copulatory behavior[J]. BMC Complement Altern Med, 2015, 15: 331.
[10] ADEJUYITAN J A. Tigernut processing: its food uses and health benefits[J]. Am J Food Technol, 2011, 6(3): 197-201.
[11] 蔡铁全, 吕晶, 高晓新, 等. 小球藻片对大鼠的致畸试验[J]. 癌变·畸变·突变, 2017, 29(1): 70-73, 77.
[12] 刘欢, 梁菲, 李莹, 等. 人参培养须根的大鼠致畸试验[J]. 癌变·畸变·突变, 2022, 34(6): 459-462.
[13] LI G, YANG L, ZHU Z, et al. Study on preparation of biodiesel from cyperus oil[J]. China Grease, 2012, 37: 59-62.
[14] CHUKWUMA E R, OBIOMA N, CHRISTOPHE O I. The phytochemical composition and some biochemical effects of Nigerian tigernut (Cyperus esculentus L.) Tuber[J]. Pakistan J Nutrition, 2010, 9(7): 709-715.
[15] ADEBAYO S F, ARINOLA S. Effect of germination on the nutrient and antioxidant properties of tigernut (Cyperus esculentus)[J]. J Biol Agric Healthc, 2017, 7: 88-94.
[16] OGUNLADE I, BILIKIS A A, OLANREWAJU G. Chemical compositions, antioxidant capacity of tigernut (cyperus esculentus) and potential health benefits[J]. Eur Sci J, 2015, 11: 217-224.
[17] VIUDA-MARTOS M, LóPEZ-MARCOS M C, FERNáNDEZ-LóPEZ J, et al. Role of fiber in cardiovascular diseases: a review[J]. Compr Rev Food Sci Food Saf, 2010, 9: 240-258.
[18] SAHEED S, OLADIPIPO A E, ABRAHAM B F. Four weeks daily dose oral administration assessment of Cyperus esculentus L. aqueous extract on key metabolic markers of wistar rats[J]. Pharmacologia, 2016, 7(2): 125-133.

油莎豆对大鼠的致畸作用研究

Teratogenicity assessment of Cyperus esculentus on rats

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