体外肝脏/肝胆类器官技术及其应用的研究进展

doi:10.3969/j.issn.1004-616x.2024.06.012

摘要/Abstract

摘要： 传统体外研究系统如细胞系、原代细胞、组织切片等在模拟体内环境方面存在局限。类器官技术利用干细胞在体外形成三维结构，能够更好地模拟真实器官的复杂结构和微环境，为现代科研提供了一种新的研究平台。药物肝毒性研究在新药研发及药物上市后安全性评价中非常重要，肝胆类器官能够更全面地模拟肝脏内多种细胞类型和微环境，为药物肝毒性研究提供了新的手段。尽管类器官技术面临扩增难度大、无法完全复制体内环境等挑战，但通过改进培养条件、结合生物材料和基因编辑技术等方法有望实现突破。本文对肝脏/肝胆类器官技术的发展，肝胆类器官的构建、培养方法、评价标准，以及将其应用在药物肝毒性评估中的研究实例等进行了综述。

关键词: 类器官技术, 体外研究模型, 肝胆类器官培养, 药物肝毒性, 药物开发

中图分类号:

Q813.1+3

徐嘉琪, 孙震晓. 体外肝脏/肝胆类器官技术及其应用的研究进展[J]. 癌变·畸变·突变, 2024, 36(6): 497-501,505.

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