Carcinogenesis, Teratogenesis & Mutagenesis ›› 1997, Vol. 09 ›› Issue (1): 28-31.doi: 10.3969/j.issn.1004-616x.1997.01.009

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DETECTION OF CYTOMEGALOVIRUS AND TOXOPLASMA GONDII INFECTION IN FETAL TISSUES OF ABORTION AND STILLBIRTHS

Shang Shiqiang, Hong Wenlan, Shi Yifu, etal   

  1. Zhejiang Medical U niversity, H angzhou, 310003
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-01-30 Published:1997-01-30

Abstract: Human cytomegalovirus (HCMV) and Toxoplasma gondii (Tox) were detected intissues of abortions and stillbirths Diagnosticmethods involved virus isolation, nested PCR,restriction endonucleases analyses of HCMV and the etiological isolation of Tox- trophozoites through m ice inoculation and single PCR to Tox The results showed, that among28 stillbirths and abortions HCMV DNA was found in the lung tissue of one fetal death ,which was negative in virus isolation. Tox DNA was positive in all tissues of a stillbirth including the heart, lungs, brain, liver, kidney ,and eye tissues Tox tachyziotes was isolated from the brain ,lung ,eye and liver tissues in the fetal death through animal inocuation but negative resultswere seen in the heart and kidney .A ll these suggests that some abortions and stillbirths result from fetal infection w ith HCMV or Tox, PCR, being more sensitive and rapid than pathogen isolation, m ight be useful for early diagnosis of abortions and stillbirths w ith HCMV or Tox infection.

Key words: Cytomegalovirus, Toxoplasma PCR, Stillbirth