Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (5): 278-281.doi: 10.3969/j.issn.1004-616x.1998.05.003

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THE SELECTION AND THE APPLICATION OF EXPERIMENTAL PARAMETERS ABOUT FLUORMETRY ASSAY OF DNA UNWINDINGON CHL CELLS

Xue Lijun , J in Zhoungchu   

  1. Dept . of Pathophysiology , Zhejiang Medical University , Hangz hou 　310031
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-09-30 Published:1998-09-30

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Abstract: To make certaion stable experimental parameters about FADU in detection of CHL DNA breaks , temperature of DNA unwinding and concent ration of unwinding alkaline solution NaOH were modified through FADU on CHL cells. According to Birnboim’s criterion , unwinding temperature first 0 ℃/ 30 min →then 5 ℃/ 60 min and NaOH 0. 4N were selected as better conditions. The reliability of these experimental parameters was established by detection of peroxidative adaptive response and antiperoxidation on CHL DNA breaks induced by H2O2 with the improved FADU.

Key words: CHL cells, FADU, temperature of unwinding, concent ration of unwinding alkaline solution, peroxidative adaptation, antiperoxidation