Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (6): 341-344.doi: 10.3969/j.issn.1004-616x.1998.06.003

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p53 GENE MUTATION OF PERIPHERAL LYMPHOCYTES IN PATIENTS WITHLARGE BOWEL CARCINOMA AND THEIR FAMILIAL MEMBERS

Li Minglie1 ,Sun Zhaozhong2 ,Zhang Yuexing1 　et al   

  1. 1S hanghai Cancer Instit ute , S hanghai 　200032 2S hanghai Medical University Cancer Hospital , S hanghai 　200032
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-11-30 Published:1998-11-30

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Abstract: Using PCR2SSCP silver staining technique , 20 patient s with large bowel cancer (LBC) ,and 20 their familial members ( FM) were analysed for mutation in exons 5 - 8 of p53 gene of peripheral lymphocytes. Among them ,p53 mutation were found in 6 cases with LBC and in 5 cases with FM. The mutation rates were 30 % and 25 % in LBC and FM ,respectively. It was showed that p53 gene mutation is a common event in LBC , which plays an important role in the progression of LBC. Our preliminary study indicates the detecting of p53 gene mutation may be used as a molecule diagnosis for susceptible individuals of LBC.

Key words: Large bowel carcinoma, Familial members, Lymphocyte, Mutation of p53 gene