Abstract: To elucidate the expression of Wilms’tumor gene (WT1) in various types of leukemia and its clinical implication. WT1 mRNA was detected in K562 cell line , 23 leukemia patient s (12AML patient , 3 ALL patient , 7 CML patient , 1 CLL patient . ) 2 lymphoma patient s in three stage , and 10 healthy subjects by using RT2PCR. WT1 mRNA expression was revealed in K562 cell line , 10 of 12 AML patient s , 2 of 3 ALL patients , 6 CML patient s in blast crisis. WT1 mRNA was undetecatable in 1 CML patient in accelerated , 1 CLL patient , 2 NHL in three phase and 10 healthy subject s. WT1 gene is expressed in early hematopoietic cells , it is closely related with acute leukemia , and supposed to be a new marker for monitoring MRD in acute leukemia.

Key words: leukemia, Wilm’s tumor gene, Polymerase chain reaction