双色荧光原位杂交检测正常人精子9 、18 号染色体非整倍体率

doi:10.3969/j.issn.1004-616x.2002.02.004

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2002, Vol. 14 ›› Issue (2): 80-083.doi: 10.3969/j.issn.1004-616x.2002.02.004

Previous Articles Next Articles

DETECTION OF ANEUPLOIDIES FOR CHROMOSOME 9 , 18 IN SPERM OF NORMAL MALES USING TWO-COLOR FLUORESCENCE IN SI TU HYBRIDIZATION

LI Xin1; ZHENG Lu-kang2; DENG Li-xia2; ZHANG Qiao2

1. Lab. Of Genetic Toxicology, Center for Disease Control and Prevention of Guangdong Province,Guangzhou 510300, China; 2. Lab. Of Genetic Toxicology , School of Public Health, Sun Yat-Sen University of Medical Sciences, Guangzhou 510089

Received:2001-07-24 Revised:2001-08-27 Online:2002-04-30 Published:2002-04-30
Contact: LI Xin

Abstract

Abstract: Purpose : To detect the f requencies of aneuploidy for chromosome 9 and 18 in sperm of healthy males. Methods : Two-color fluorescence in situ hybridization ( FISH) was performed on sperm nuclei using cent romeric probes for chromosome 9 and 18 , and the f requencies of aneuploidy were scored. Results : 156 955 sperm nuclei were detected in 16 healthy adults. About 10 000 sperm nuclei were scored from each of 16 donors and the average efficiency of hybridization was above 99 %. The mean f requencies of disomy obtained were 0. 050 % ±0. 030 % for chromosome 9 ,0. 033 %±0. 025 %for chromosome 18. The nullisomy frequencies of chromosome 9 and 18 were found to be 0. 067 %±0. 037 % , 0. 048 %±0. 034 % respectively. Diploidy was observed as 0. 040 %±0. 036 %. The f requency of total numerical aberration was 0. 218 %±0. 071 %. Con-clusion : The aneuploidy for chromosome 9 and 18 in sperm of 16 healthy males was detected in this research , the result was similar to that of traditional sperm karyotype analysis.

Key words: fluorescence in situ hybridization, aneuploidy, spermatozoa

CLC Number:

Q343

LI Xin, ZHENG Lu-kang, DENG Li-xia, ZHANG Qiao. DETECTION OF ANEUPLOIDIES FOR CHROMOSOME 9 , 18 IN SPERM OF NORMAL MALES USING TWO-COLOR FLUORESCENCE IN SI TU HYBRIDIZATION[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2002, 14(2): 80-083.

[1]	LI Penghao, QU Ting, HUANG Jihua, HAN Tingting, WEN Zina, CUI Shuyan, ZHONG Ying. A refined technique for fluorescence in situ hybridization with human oocyte chromosomes [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2016, 28(2): 131-133,140.
[2]	NI Juan，ZOU Tian-ning，WANG Xu. The effect of folate and riboflavin on chromosomes 8 and 17 aneuploidy in lymphocytes of breast cancer patients and normal controls [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2013, 25(1): 26-30.
[3]	TANG Yong-fei;ZHAN Na;HUANG Ya-bing;LIU Lin. Clinicopathological implication of HER-2/neu protein expression and gene amplication in esophageal carcinoma [J]. , 2012, 24(4): 256-260.
[4]	WANG Dian, KANG Xiang-jin, XIE Qing-dong, HUANG Tian-hua, . The expression of human immunodeficiency virus _1 gag gene in mature human spermatozoa [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(6): 455-457.
[5]	ZHANG Qiu-ju, HUANG Tian-hua, XIE Qing-dong , TAN Xiao-fang, LIU Ge-fei, CHEN De-yu, ZHOU Xiao-ling. Protein Expression of Human Spermatozoa-mediated HBs Gene and HBc Gene in Early Embryonic Cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2008, 20(3): 185-188.
[6]	MA Ming-fu, LI Lian-bing, LU Jing, LI Xin-sheng, CHEN Xiao-xing, ZHAO Le-tian. Aneuploidy Detection by Multi-color Fluorescence in Situ Hybridization in Patients with Asthenospermia [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2008, 20(3): 239-241.
[7]	CAO Bo, WEN Sha_luo. Study on Centromeric Dots Variation in JEG_3 Cells and PA_1 Cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2008, 20(1): 64-067.
[8]	HUANG Ji-hua, YANG Fang-ju. Effects of Haloperidol on Mouse Oocyte Chromosomes [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2005, 17(3): 144-147.
[9]	CAI Min, LI Guang-kun, LI Shu-jing. Analysis on Chromosome Aberration of Cryopreservative Human Spermatozoa [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2005, 17(1): 22-24.
[10]	CAO Bo, WANG Yin-xiong. Study on Centromeric Dots Variation of HepG2 Cells [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2004, 16(6): 335-337.
[11]	RUAN Ye, WANG Xiao-yun, XIE Qing-dong, et al. Experimental Study on Sperm-mediated Foreign DNA Transfer [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2004, 16(3): 144-147.
[12]	LIU Xin -xia, HUANG Guo-xian, DENG Li-xia, et al. Simultaneous Detection of the Numerical and Structural Chromosome Aberrations of Sperm in Normal Men by Multi－color Fluorescence in situ Hybridization (FISH) [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2004, 16(1): 9-12.
[13]	LI Hua, LIU Wen, LI Jin-yan, et al.. EFFECT OF LOW DOSE IONIZING RADIATION ON HUMAN LYMPHOCYTES CHROMOSOME NON-DISJUNCTION [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2003, 15(2): 67-71.
[14]	DING Yin-run, WANG Xiao-yan, WANG Xu. ANEUPLOIDY INDUCTION BY THE WATER EXTRACT OF TRI PTERYGIUM HYPOGLAUCUM ( LEVEL ) HUTCH IN MOUSE BONE MARROW CELLS DETECTED BY FISH [J]. , 2002, 14(3): 144-146.
[15]	SHUAI Zu-bing, HUANG Tian-hua, ZENGLi-ping, HUANGJian-min, SUN Xi-hai. THE ANIMAL MODEL FOR INSPECTION OF ANEUPLOIDY IN PREIMPLANTATION EMBRYOS OF MICE [J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2002, 14(1): 19-022.

DETECTION OF ANEUPLOIDIES FOR CHROMOSOME 9 , 18 IN SPERM OF NORMAL MALES USING TWO-COLOR FLUORESCENCE IN SI TU HYBRIDIZATION

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments