Abstract: Purpose : To address closely the effect of fibrin on the development of mouse embryo invit ro. Methods : Two2cell mouse embryos were divided into two random groups : Group 1 (experiment group) , with fibrin glue ; Group 2 (cont rol group) , without fibrin glue. Embryo development and cleavage of the two groups were compared every day for 5 days. The chromosome analysis of hatched embryo cells were also performed. Results : The percentages of embryos reaching blastocyst/ hatching stages were as follows : Group 1 , 67. 5 % and 38. 5 % respectively ; Group 2 , 66. 7 % and 34. 0 %( P > 0. 05 , Group 1 vs Group 2) , respectively. The percentages of normal chromosome number ( diploid) in two groups were 92 % and 90 % , respectively ( P = 0. 387 , Group 1 vs Group 2) . Conclusion : There were no significant difference between two groups on the blastocyst/ hatching development rates and chromosome analysis. These data demonst rate that fibrin glue have no toxicity on mouse embryo development and are suitable for interaction study between the embryot rophic factor and development embryos.

Key words: fibrin, embryo development, vehicle