抗肿瘤药物体外诱导新鲜癌组织及其外周血淋巴细胞mdr-1 基因表达的相关性研究

doi:10.3969/j.issn.1004-616x.2002.04.007

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2002, Vol. 14 ›› Issue (4): 229-230.doi: 10.3969/j.issn.1004-616x.2002.04.007

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STUDY ON THE RELATIVITY OF mdr-1 EXPRESSION INDUCED BY DRUG BETWEEN FRESH TUMOUR CELLS AND THE PERIPHERAL LYMPHOCYTES IN VITRO

HE Lin1, ZHANG Qiao2, HU Jie-ying1

1. Department of Molecular Biology, Henan Tumour Institute, Zhengzhou 　450003, China; 2. School of Public Health, Zhengzhou University, Zhengzhou 450052, China

Received:2002-03-14 Revised:2002-05-21 Online:2002-10-30 Published:2002-10-30

Abstract

Abstract: Purpose : To investigate the relativity of mdr-1 expression between the f resh tumour cells and the peripheral lymphocytes of 32 patients with malignant tumours. Methods : Using reverse t ranscription2polymerase chain reaction (RT-PCR) assay , mdr-1 expression induced by 8 anticancer drugs in the f resh tumour cells and the peripheral lymphocytes of 32 patients with malignant tumours was detected. Results : There was a positive relativity of mdr21 expression between the fresh tumour cells and the lymphocytes. Conclution : The lymphocytes of peripheral blood can replace tumour cells in selecting sensitive drugs with RT-PCR assay invit ro. It can provide a new way of using drug individually for patients with malignant tumours.

Key words: tumour cell, lymphocyte, RT-PCR, mdr-1

CLC Number:

R737236

HE Lin, ZHANG Qiao, HU Jie-ying. STUDY ON THE RELATIVITY OF mdr-1 EXPRESSION INDUCED BY DRUG BETWEEN FRESH TUMOUR CELLS AND THE PERIPHERAL LYMPHOCYTES IN VITRO[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2002, 14(4): 229-230.

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STUDY ON THE RELATIVITY OF mdr-1 EXPRESSION INDUCED BY DRUG BETWEEN FRESH TUMOUR CELLS AND THE PERIPHERAL LYMPHOCYTES IN VITRO

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