Carcinogenesis, Teratogenesis & Mutagenesis ›› 2003, Vol. 15 ›› Issue (1): 13-16.doi: 10.3969/j.issn.1004-616x.2003.01.004

Previous Articles     Next Articles

CONSTRUCTION AND IDENTIFICATION OF THE BAIT PLASMIDS pHis-3NF AND pLacZ-3NF FOR YEAST ONE-HYBRID SYSTEM

NIU Yong-dong 1, XU Li-yan 2, LI En-min 1   

  1. 1. Department of Biochemistry ＆ Molecular Biology; 2. Insititue of Oncologic Pathology,Medical College of Shantou University, Shantou 515031 , China
  • Received:2002-10-31 Revised:2002-11-26 Online:2003-01-30 Published:2003-01-30
  • Contact: LI En-min

PDF (PC)

2784

Abstract: Purpose: To construct the pHis-NF and pLacZ-NF plasmids which could be integrated into the yeast genome. Methods: the target DNA sequence of tandem NF-κB copies was synthesized, and the tandem copies were directionally inserted into the MCS of E.coli yeast shuttle pHisi and pLacZi plasmid vectors respectively. The recombinant were confirmed by restriction enzyme digestion, agarose gel, PAGE electropheresis and DNA sequencing. Results: Both PAGE electropheresis and DNA sequence analysis confirmed the inserted oligonucleotides. Conclusion: These two plasmids, pHis-3NF and pLacZ-3NF have been successfully constructed.

Key words: Nuclear factors, NF-κB, pHis-3NF, pLacZ-3NF, Yeast one-hybrid system