电离辐射调控脂质体介导的TNFα基因在HeLa细胞中的表达

doi:10.3969/j.issn.1004-616x.2004.02.007

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2004, Vol. 16 ›› Issue (2): 89-91.doi: 10.3969/j.issn.1004-616x.2004.02.007

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Ionizing Radiation Regulated Expression of HeLa Cells by Lipofectamin－Mediated TNF α Gene

LIU Jian-xiang;SU Xu

National Institute for Radiological Protection, China CDC, Beijing 100088，China

Received:2004-01-09 Revised:2004-02-11 Online:2004-03-30 Published:2004-03-30
Contact: LIU Jian-xiang

Abstract

Abstract: BACKGROUND ＆ AIM : To investigate the expression of p－Egr－TNFα, cell cycle and apoptosis in HeLa cells, which were transfected by recombined plasmid p－Egr－TNFα, induced by 60Co γ－rays irradiation in different doses. MATERIAL AND METHODS: The expression of TNFα was detected by ELISA. The cell cycle and apoptosis were detected by Flow Cytometry. RESULTS: After γ－rays irradiation in different doses, the expression of TNFα was(250～400)pg/ml,significantly higher than that of the control group. The percentages of S and G2 M phase were markedly higher than that of the control group,and showed a dose－dependent increase, the rate of G0/G1 showed a dose－dependent decrease. Cell apoptosis rate showed a dose－dependent increase. CONCLUSION: γ－rays can regulate expression of p－Egr－TNFα by Lipofectamin－mediated TNFα gene in HeLa cells. The expression level shows a significantly increase in transfected HeLa cells. The change of cell cycle and apoptosis shows a dose－dependent effect.

Key words: 60Co γ－rays, TNFα expression, cell cycle, dose effect

LIU Jian-xiang, SU Xu. Ionizing Radiation Regulated Expression of HeLa Cells by Lipofectamin－Mediated TNF α Gene[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2004, 16(2): 89-91.

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Ionizing Radiation Regulated Expression of HeLa Cells by Lipofectamin－Mediated TNF α Gene

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