›› 2005, Vol. 17 ›› Issue (1): 15-18.doi:

• 论著 • Previous Articles     Next Articles

Effects of 60Co-γ Ray Local Irradiation on Proteins of Bone Marrow in Tumor-bearing Rats

LI Bo, HAI Chun-xu, QIN Xu-jun, et al   

  1. Department of Toxicology, Faculty of Preventive Medicine,the Fourth Military Medical University, Xi'an 710032,China
  • Received:2004-07-23 Revised:2004-10-25 Online:2005-01-30 Published:2005-01-30
  • Contact: HAI Chun-xu

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Abstract: BACKGROUND ＆ AIM: To observe the diffenential expression of proteins and the index of oxidative injury of bone marrow after 60Co-γray local irradiation in tumor-bearing rats. MATERIAL AND METHODS: The entitative tumor pieces were embedded into hind leg muscle of rat to prepare tumor bearing rat model. After 60Co-γray local irradiation，the bone marrow of rats in both non-irradiated and irradiated groups were removed respectively, to detect the MDA, GSH, GSSH of bone marrow, and analyze the differential expression of bone marrow proteins by using two-dimensional polyacrylamide gel electrophoresis. RESULTS: The levels of alterations of 24 proteins in irradiated group, including 2 new proteins, 4 down-regulated proteins, 1 up-regulated protein and 17 proteins disappeared. CONCLUSION: 60Co-γray local irradiation can cause differentially expressed proteins of bone marrow in tumor bearing rats, and oxidative injury may be an important factor for differentially expressed proteins.

Key words: irradiation, oxidative injury, proteins, two-dimensional polyacrylamide gel electrophoresis, bone marrow