Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (1): 4-7.doi: 10.3969/j.issn.1004-616x.2005.01.002

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Protection Effects of Ribonuclease Inhibitor onthe Cells Peroxidative Damage Injured by H2O2

XIA Jun;YAN Ping;CHEN Jun-xia;et al   

  1. Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116027, China
  • Received:2004-03-08 Revised:2004-06-16 Online:2005-01-30 Published:2005-01-30
  • Contact: CUI Xiu-yun

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Abstract: BACKGROUND ＆ AIM: Ribonuclease inhibitor (RI) is an acidic cytosolic glycoprotein. It has plenty of reduced thiol groups. According to this point, we study the effects of RI on the melanoma B16 cells injured by H2O2. MATERAIL AND METHODS: The transfected B16 cells with RI cDNA in which RI was overexpressed and the control B16 cells were treated with different dose of H2O2 respectively. The different changes of cell viability, LDH leakage, MDA contents and the activities of antioxidative enzymes were investigated by comparison between these two kinds of cells. RESULTS：The transfected B16 cells had higher viability，less LDH leakage and MDA contents，and higher activities of GSHPx，SOD and CAT. CONCLUSION： This result showed that RI had antioxidant activity and it was able to protect cells from peroxidative injuries.

Key words: ribonuclease inhibitor, melanoma B16 cells, hydrogen peroxide, antioxidant activity