Abstract: BACKGROUND ＆ AIM: The carcinogenesis of the human cervical precancerous lesion,cervical carcinoma is known closely associated with human papillomavirus(HPV).The purpose of this study was is to identify whether Herpes Simplex Virus(HSV) and Cytomegalovirus(CMV) play a co-factor role in the carcinogenesis. MATERIAL AND METHODS: Eighty-one cases of various cervical lesions were analyzed by HPV6/11,HPV16/18 in situ hybridization. Meanwhile, HPV,HSV and CMV were determined in 103 cases of various cervical lesions. RESULTS: The results show that the distribution of positive hybridization signal was consistent with the distribution of koilocytes in HE stain. Of these cervical specimens investigated, the positive rates of HPV 16/18 and HPV6/11 using ISH were 51.1 ％ and 64.7 ％, respectively,the infection rates of HPV 16/18, HPV 6/11,HSV and CMV using PCR were 21 ％,4 ％,23 ％ and 0 ％,respectively. CONCLUSION: The co-operation effect of HPV and HSV might occur in the oncogenesis of human cervical carcinoma.

Key words: cervical carcinoma, human papillomavirus, in situ hybridization