Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (5): 298-301.doi: 10.3969/j.issn.1004-616x.2005.05.012

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Detection of DNA Damage Induced by Methotrexate in Multiple Mouse Organs Using SCGE Assay

YANG Jian-yi, PENG Yun, LI Li, DU Sheng-jia,SHAN Lian-zhe,ZHANG Xin-wang,WANG Wen-juan   

  1. School of Basic Medical Science,Shanxi Medical University,Taiyuan 030001,Shanxi,China
  • Received:2004-06-09 Revised:2004-08-28 Online:2005-09-30 Published:2005-09-30
  • Contact: YANG Jian-yi

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Abstract: BACKGROUND ＆ AIM: In order to understand the mechanism of methotrexate (MTX) further and to investigate the genotoxic target organs.MATERIAL AND METHODS： DNA damage and the correlation with dosage treated with MTX were studied by using the alkaline single cell gel electrophoresis assay(SCGE). Liver, spleen, bone marrow, thymus, kidney, testicle, stomach and peripheral lymphocytes of mice were isolated at 1、3、6、12、24 h after MTX intraperitoneal injection. RESULTS: Significant increase in DNA migration and comet frequency in the spleen, thymus, bone marrow and peripheral lymphocytes were induced after intraperitoneal treatment of MTX at a dose of 1.25～5 mg/kg. The migration of nuclear DNA and comet frequency of spleen, thymus, bone marrow and peripheral lymphocytes in the dose-response study showed a dose-dependent increase. CONCLUSION: The results indicate that DNA SSBs could be induced by MTX in some cells of mice. There are difference in sensitivity of various organs in the mice and cells of spleen, thymus, bone marrow and peripheral lymphocytes may be the important target cells of MTX.

Key words: methatrexate, single cell gel electrophoresis, DNA damage, target organ