Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (2): 135-138.doi: 10.3969/j.issn.1004-616x.2006.02.016

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The Design and Fabrication of Mouse Toxicology Gene Chip

AO Lin1; ZENG Zhi-xiong2; FANG Zhi-jun2; HU Ran1;GAO Li-hong1; YANG Meng-shu2;CAO Jia1   

  1. 1.Department of Toxicology,College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China；2. Applied Research Center for Genomic Technology, City University of Hong Kong, Hong Kong, China
  • Received:2005-10-08 Revised:2005-12-02 Online:2006-03-30 Published:2006-03-30
  • Contact: AO Lin

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Abstract: BACKGROUND ＆ AIM： To design and fabricate a kind of mouse toxicology gene chip to be used in toxicology research. MATERIAL AND METHODS： Some genes related to different biological functions were chosen according to the requirement of environmental and toxicology research. NIA mouse cDNA clone sets were applied to copy and amplify the gene fragments which were dotted on the slide, and the Picogreen fluorescence staining was used to detect the quality of chip. RESULTS： We chose 1 796 genes involved in eight biological functions for the construction of the mouse toxicology gene chip. 95.12％ of the PCR products had strong and single band, and the slides selected from each batch had good images with Picogreen dye detection. CONCLUSION： We have designed and fabricate the mouse toxicology. gene chip successfully. This could be applied in toxicology-related research.

Key words: gene chip, toxicology

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