Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (5): 400-403.doi: 10.3969/j.issn.1004-616x.2006.05.020

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DNA Damage Induced by UV in K562 Cells Measured by Comet Assay

LUO Ming-zhi1,2, QI Hao1, CHEN Wen-fang1, LIU Miao1, GUO Wei1   

  1. 1.College of Life Science, Shanxi Normal Univercity, Xi'an 710062;2.Departmemt of Life Science, Xi'an University of Art and Science，Xi'an 710065
  • Received:2005-05-12 Revised:2006-03-21 Online:2006-09-30 Published:2006-09-30
  • Contact: QI Hao

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Abstract: BACKGROUND ＆ AIM： This article exploved a modified method of comet assay from the Singh's alkaline comet assay and discussed the reliability of this method and the CASP software used to measure the DNA damage induced by UV light． MATERIAL AND METHODS： DNA damage of K562 cells induced by UV for different times (3 s,5 s,10 s,40 s,60 s,120 s,180 s,240 s) was measured by comet assay．The results were assayed by CASP． RESULTS： UV( 0.3 mW/cm2)induced a significant DNA damage (P<0.01) when the results were assayed by the four parameters (tail length，comet length，tail moment，olive tail moment)． CONCLUSION： The results suggested that the four parameters indicated a good time_dependent effect.The method which we developed and the four parameters were reliable．

Key words: DNA damage, single cell gel electrophoresis, Ultraviolet, K562