甲基硝基亚硝基胍诱导CHL细胞中γH2AX焦点的形成

doi:10.3969/j.issn.1004-616x.2006.06.001

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (6): 417-419.doi: 10.3969/j.issn.1004-616x.2006.06.001

N-methyl-N-nitro-N-nitrosoguanidine Induces the Phosphorylation of Histone H2AX in CHL Cells

SHI Wei , YU Yan-ke, ZHU Xin-qiang , YANG Jun

(1.Department of Pathology ＆ Pathophysiology, Zhejiang University, School of Medicine; 2. Institute of Toxicology, Zhejiang University, School of Public Health Hangzhou, Zhejiang 310058, China)

Received:2006-04-18 Revised:2006-07-11 Online:2006-11-30 Published:2006-11-30
Contact: SHI Wei

Abstract

Abstract: BACKGROUND＆AIM: To study whether N-methyl-N＇-nitro-N-nitrosoguanidine(MNNG) could induce the formation of γH2AX foci in CHL cells. MATERIALS AND METHODS: The cytotoxic effects of different concentrations of MNNG were evaluated by MTT test. The phosphorylation of H2AX was assessed by using immunofluorescent microscopy. RESULTS: MNNG exhibited a dose- and time-dependent cytotoxic effect on CHL cells. MNNG treatment(1 mg/L) significantly increased the ratio of γH2AX-positive cells and the number of foci/cell at 2, 8, and 24 h. The ratios of cells with over 30 foci/cell were about 56％,92％ and 91％, respectively. CONCLUSION: MNNG could induce the phosphorylation of H2AX in CHL cells in a time-dependent manner.

Key words: MNNG, γH2AX, DNA damage, genotoxin

CLC Number:

R994.6

SHI Wei , YU Yan-ke, ZHU Xin-qiang , YANG Jun. N-methyl-N-nitro-N-nitrosoguanidine Induces the Phosphorylation of Histone H2AX in CHL Cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2006, 18(6): 417-419.

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N-methyl-N-nitro-N-nitrosoguanidine Induces the Phosphorylation of Histone H2AX in CHL Cells

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