Abstract: BACKGROUND ＆ AIM:To investigate the effects and mechanisms of resveratrol on human colon cell line HT_29. MATERIALS AND METHODS: HT_29 cells were incubated with 0,12,5,25,50,100 μmol/L resveratrol. The proliferation of HT_29 cells was measured by MTT,the COX_2 were analyzed by Western blot,the Bcl_2 and Bax were detected by immunohistochemistrical method. RESULTS:MTT results revealed that resveratrol could inhibit the proliferation of HT_29 cells in a dose and time_dependent manner.And after HT_29 cells were treated with different concentrations of resveratrol for 48 h, resveratrol at concentrations of 50 and 100 μmol/L could inhibit the expression of COX_2,and resveratrol at concentrations of 100 μmol/L could induce the expression of Bax. CONCLUSION: Resveratrol could inhibit the proliferation of HT_29 cells in a dose and time_dependent manner. The inhibition of COX_2 protein expression and the induction of Bax protein expression may be the underlying mechanisms.

Key words: resveratrol, human colon cell line