Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (4): 258-262.doi: 10.3969/j.issn.1004-616x.2009.04.003

Previous Articles     Next Articles

Over-expression of ATF3 Inhibits the Proliferation of Esophageal Squamous Carcinoma Cells

XIE Yang-min1; XIE Jian-jun2;#; ZHOU Fei1;2;#;HOU Jian2; CAO Jun-jun1; XU Li-yan3; LI En-min2;   

  1. 1. Department of Experimental Animal Center, Medical College of Shantou University, Shantou 515041; 2. Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041; 3. Department of Pathology, The Key Immunopathology Laboratory of Guangdong Province, Medical College of Shantou University, Shantou 515041,China
  • Received:2009-05-22 Revised:2009-05-27 Online:2009-07-30 Published:2009-07-30
  • Contact: LI En-min

PDF (PC)

2132

Abstract: BACKGROUND AND AIM: ATF3 was down-regulated in esophageal squamous cell carcinoma (ESCC), but the roles of ATF3 in ESCC cells still remained unclear. The purpose of this study was to explore the effect of ATF3 on the proliferation of ESCC cells. MATERIALS AND METHODS: The recombinant expressing plasmid was constructed by inserting the full coding sequence of ATF3 gene into the eukaryotic expressing vector pcDNA3. Then, the expressing plasmid was stably transfected into EC109 cells, an ESCC cell line, and the over-expressing ATF3 cell clones were obtained. Colony formation assay and tumor formation assay in nude mice were used to explore the effect of ATF3 over-expression on the proliferation of ESCC cells. RESULTS: With the over-expression of ATF3, the colony formation ability of EC109 cells was decreased and the tumor formation of EC109 cells in female mice was inhibited. CONCLUSION: Over-expression of ATF3 could inhibit the proliferation of ESCC cells and ATF3 may play important roles in the progression of ESCC.

Key words: esophageal squamous carcinoma, activating transcription factor 3, cell proliferation

CLC Number: