Carcinogenesis, Teratogenesis & Mutagenesis ›› 2009, Vol. 21 ›› Issue (6): 418-421.doi: 10.3969/j.issn.1004-616x.2009.06.003

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Effect of Sphingomyelin Hydrolysates on Alkaline Sphingomyelinase Expression in Human Cancer HT-29 Cell

ZHANG Ping1; YUAN Li-jie1; ZHAO Lei1; LI Bai-xiang2   

  1. 1.Department of Biological Chemistry, Daqing Campus of Harbin Medical University, Daqing 163319, Heilongjiang; 2. Department of Toxicology, Public Health College, Harbin Medical University, Harbin 150081, Heilongjiang, China
  • Received:2009-05-20 Revised:2009-06-15 Online:2009-11-30 Published:2009-11-30
  • Contact: ZHANG Ping

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Abstract: BACKGROUND AND AIM: Alkaline sphingomyelinase(alk-SMase) is the key enzyme of sphingomyelin(SM) metabolism, and an inhibitor of colon cancer. Through vitro experiments, we examined the alk-SMase expression in human colon cancer HT-29 cells, which produced the hydrolysis products of SM, ceramide(Cer) and sphingosine(Sph). MATERIALS AND METHODS: After treatment with 12.5,25 and 50 μmol/L of C2-Cer and Sph for 12,24 and 48 hours, we tested the alk-SMase mRNA and protein expressions in HT-29 cells. DMSO was the control. RESULTS: Compared with the control group, the alk-SMase expression of protein and mRNA level was reduced by C2-Cer and Sph in HT-29 cells, With a clear dose-response relationship. CONCLUSION: Cer and Sph exerted negative feedback on alk-SMase. Cer and Sph might have induced apoptosis directly in colon cancer cells, rather than through hydrolysis of SM.

Key words: ceramide, sphingosine, alkaline sphingomyelinase, colon cancer HT-29 cells

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