Carcinogenesis, Teratogenesis & Mutagenesis ›› 2010, Vol. 22 ›› Issue (2): 81-085.doi: 10.3969/j.issn.1004-616x.2010.02.001

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SET expression alteration in L-02 liver cells treated with trichloroethylene

JIANG Ying-zhi1;2;LIU Jian-jun2;ZHOU Gui-feng1;ZHANG Bing2;ZHENG Bo2;HUANG Ai-jun2   

  1. 1. Department of Preventive Medicine,The Medical College of Hunan Normal University, Changsha 410006, Hunan; 2. Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, Guangdong，China
  • Received:2009-10-30 Revised:2009-12-01 Online:2010-03-30 Published:2010-03-30
  • Contact: LIU Jian-jun

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Abstract: OBJECTIVE: To investigate the effects of different doses of trichloroethylene(TCE) on the expression alteration of SET gene at both the mRNA and protein levels, in order to provide some clues for the liver-injury mechanism of TCE. METHODS: Real-time quantitative PCR and Western blotting were employed to detect the mRNA and protein levels of SET in L-02 liver cells, which were separately treated with DMSO, 3 mmol/L and 10 mmol/L TCE. RESULTS: Compared with the control group, the mRNA and protein levels of SET were both significantly up-regulated (P<0.01) in the L-02 liver cells treated with TCE, and the expression of SET protein in the 10 mmol/L TCE group was higher than 3 mmol/L TCE group, showing a dose-dependent relationship. CONCLUSION: TCE could induce the expression of SET at both the mRNA and protein levels in a dose-dependent manner, which indicated that SET might play an important role in the hepatotoxicity of TCE.

Key words: trichloroethylene, SET, Western blotting, real-time quantitative PCR

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