Abstract: OBJECTIVE: To determine the transfection expression of plasmid pcDNA3.1-IDO in human hepatoma carcinoma cell line hepG2, and to establish a transfection method of hepG2. METHODS: The plasmid pcDNA3.1-IDO was amplified in Escherichia coli. The cultured hepG2 cells were transfected with pcDNA3.1-IDO by lipofectamineTM2000 reagent. The hepG2 cells and hepG2 cells trancfected with blank plasmid pcDNA3.1(pcDNA3.1- hepG2 cell) were used as control group. The transient expression of IDO in hepG2 cells transfected with recombinant plasmid was determined by RT-PCR and Western blot. RESULTS: IDO gene and protein were expressed transiently in hepG2 cells transfected with recombinant plasmid shown by RT-PCR and Western blot,respectively. CONCLUSION: The IDO gene was successfully transfected into human hepatoma carcinoma cell line hepG2 by means of lipofectamineTM2000 reagent, which provides the basis for further studies of IDO gene.

Key words: gene transfection, human indoleamine 2, 3-dioxygenase, hepG2 cell