›› 2011, Vol. 23 ›› Issue (2): 119-122.doi: 10.3969/j.issn.1004-616x.2011.02.009

• 论著 • Previous Articles     Next Articles

Effects of ionizing radiation on the proliferation and cell cycle of gastric adenocarcinoma cells

GAO Ling1,2, LI Feng-sheng3, CHEN Xiao-hua2, LIU Qing-jie1,*   

  1. 1. National Institute for Radiological Protection，China CDC, Beijing 100088; 2. Institution of Radiate Medicine, Academy of Military Medical Sciences, Beijing 100850; 3. General Hospital of Second Artillery, Beijing 100088, China
  • Received:2010-10-20 Revised:2010-11-20 Online:2011-03-30 Published:2011-03-30
  • Contact: LIU Qing-jie

PDF (PC)

1355

Abstract: To study the effects of ionizing radiation on the proliferation, apoptosis and cell cycle of gastric adenocarcinoma cells in vitro. METHODS: After gastric adenocarcinoma BGC-823 cells were irradiated by 60Coγ-rays with a single dose of 0, 2 or 4 Gy, the proliferation ability of BGC-823 cells in every dose group was evaluated by MTT and cloning efficiency assay. Apoptosis and cell cycle were further investigated by flow cytometry with Annexin-V/PI double staining and PI staining, respectively. RESULTS: Compared with the 0 Gy group, proliferation of gastric adenocarcinoma cells was inhibited significantly after doses of 2 and 4 Gy γ-rays irradiation (P<0.05). Furthermore, the cells were arrested in S then G2/M phases after γ-rays irradiation of 2 and 4 Gy. Finally, compared with 0 Gy group, the percentage of apoptotic cells in 4 Gy dose group increased significantly 48 h after irradiation (P<0.05). CONCLUSION: Radiation treatment of gastric adenocarcinoma BGC-823 cells with 60Coγ-rays could inhibit cell proliferation, induce cell cycle arrest and promote apoptosis.

Key words: gastric adenocarcinoma, radiation, proliferation, apoptosis, cell cycle